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Analysis of plankton by flow

Analysis of plankton by flow. cytometry. D. MARIE, D. VAULOT, F. PARTENSKY, N. SIMON. Sampling. Fresh sample. Unstained samples. Sampling. Fresh sample. Fixation. Unstained samples. Paraformaldehyde fixation. Sampling. Fresh sample. Freezing -80°C. Fixation. Stained samples.

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Analysis of plankton by flow

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  1. Analysis of plankton by flow cytometry D. MARIE, D. VAULOT, F. PARTENSKY, N. SIMON

  2. Sampling Fresh sample Unstained samples

  3. Sampling Fresh sample Fixation Unstained samples

  4. Paraformaldehyde fixation

  5. Sampling Fresh sample Freezing -80°C Fixation Stained samples Unstained samples Thawing

  6. Fluorescent dyes used for the analysis of nucleic acids of marine samples AcridineOrange Propidium iodide Ethidium bromide Chromomycin A3 Mythramycin DAPI Hoechst 33342 Hoechst 33258 BO-PRO-1 PO-PRO-1 TO-PRO-1 YO-PRO-1 BOBO-1 POPO-1 TOTO-1 YOYO-1 SYTO SYTOX Green PicoGreen SYBR Green-I SYBR Green-II OliGreen RiboGreen

  7. Bacterial Staining: SYBR Green-I

  8. Sampling Fresh sample Freezing -80°C Fixation Stained samples Unstained samples Thawing Addition of beads

  9. Sampling Fresh sample Freezing -80°C Fixation Stained samples Unstained samples Thawing Addition of beads CytoWin Cell parameters

  10. Equatorial Pacific Vaulot and Marie, 1999 JGR

  11. Equatorial Pacific Vaulot and Marie, 1999 JGR

  12. Sampling Fresh sample Freezing -80°C Fixation Stained samples Unstained samples Thawing Addition of beads CytoWin Mcycle Cell parameters Cell cycle analysis

  13. Cell cycle analysis

  14. Vaulot et al., 1995 Science

  15. Depth profile Almofront2 CTD512

  16. DNA QUANTIFICATION OF PROCHLOROCOCCUS

  17. In situ hybridization

  18. http://www.sb-roscoff.fr/Phyto/publi.html Fred

  19. http://www.sb-roscoff.fr/Phyto/publi.html Fred

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