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CAPILLARY ELECTROPHORESIS SYSTEMS

CAPILLARY ELECTROPHORESIS SYSTEMS. CAPEL. CAPILLARY ELECTROPHORESIS SYSTEMS CAPEL. Simultaneous quantitative determination of several components in a complex mixture. CERTIFICATION. ADVANTAGE S. CAPEL-SERIES. High separation efficiency Low reagent consumption Simple sample preparation

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CAPILLARY ELECTROPHORESIS SYSTEMS

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  1. CAPILLARY ELECTROPHORESIS SYSTEMS CAPEL

  2. CAPILLARY ELECTROPHORESIS SYSTEMSCAPEL Simultaneous quantitative determination of several components in a complex mixture CERTIFICATION

  3. ADVANTAGES CAPEL-SERIES • High separation efficiency • Low reagent consumption • Simple sample preparation • Compact benchtop design • Autosamplers (10 inlet and and 10 outlet vials) in CAPEL-104, CAPEL-104T, and CAPEL-105 • Implementation of various methods (Capillary Zone Electrophoresis, Micellar electrokinetic chromatography, Capillaryisoelectric focusing, capillary gel electrophoresis, capillary isotachophoresis)

  4. + + + + + + + + + + + + + + + + + + + + _ + _ + _ + _ _ + + _ + _ _ + + + + + + + + + + + + + + + + + + + + + ELECTROOSMOTIC FLOW (EOF) K+ Both anions and cations can be analysed in one run A- EOF _ + R – Si-OH  R – Si-O- + H+ pH > 3.0

  5. BASIC FEATURES CAPEL-103Р, 103РТ • wavelength254 nm • manual operation mode • liquid cooling (model 103РТ) CAPEL-104, 104Т • wavelength 254 nm • autosampler • liquid cooling (model 104Т) CAPEL-105 • wavelength 190-380 nm • autosampler • liquid cooling

  6. MAJOR APPLICATIONS Environment Pharmacology • pesticides • inorganic ions • phenols • medicines formulations • pharmacokinetics Clinical diagnostics Food • proteomics • DNA / RNA • affinity studies • immunoanalyses • preservatives • dyes • target compounds, etc. Forensic Investigations Chemical industry • drugs • explosives • lethal markers, etc.

  7. ANALYSIS OF PSYCHOTROPIC DRUGS MECK separation of carbamazepine and its main metabolites Chiral separation of carbamazepine metabolite

  8. ANALYSIS OF ANTIEPILEPTIC DRUGS Microemulsion EKC of 6 closely related antiepileptic drugs Selectivity changes are seen with different organic modifiers

  9. mAU 2 4 6 8 min ANALYSIS OF WATER – SOLUBLE VITAMINS Buffer0.01M borate, pH 9.8 Samplestandard solution of vitamins Capillaryl/L 50/60 cm, id 75 µm Injection600 mbar*s Voltage+20 kV Detection 254 nm 1– vitamin В1 2– vitamin В12 3– vitamin В2 4– vitamin В6 5– ascorbic acid 6– nicotinic acid 7– folic acid

  10. ANALYSIS OF MEDICALLY RELEVANT PEPTIDES Buffer 10 mM phosphate, pH 2.5 Capillary26 / 35 cm, 50µm ID Injection150 mbar x sec Voltage25 kV Sample 10 µg/mL each 1-antithrombine 2-bradikinine 3-angiotensin I 4-angiotensine II

  11. 0.2 mV 9 5 7 4 6 2 8 3 1 min 1 2 3 4 5 6 7 8 9 10 11 12 13 14 ANALYSIS OF THE CATION COMPOSITION OF THE PORTABLE, NATURAL AND WASTE WATER Buffer 6 mmol benzimidazole, 2.5 mmol tartaric acid, 2.0 mmol 18-crown-6 Samplecalibration solution Capillary l/L 50/60 cm, id 75 µm Injection 150 mbar*s Voltage +10 kV Detection 254 nm, indirect 1– cesium 2– ammonium 3– potassium 4– sodium 5– magnesium 6– lithium 7– strontium 8– barium 9– calcium

  12. 3 9.16 mAU 2 1 5 4 6 ** * min 1 2 3 4 5 6 ANALYSIS OF THE ANION COMPOSITION OF THEPORTABLE, NATURAL AND WASTE WATER Buffer 5 mmol chromate, 20 mmol DEA, 1.65 mmol CTAB Sample calibration solution, 5 mg/l of each anion (fluoride  2.5 mg/l) Capillary l/L 50/ 60 cm, id 75 µm Injection 300 mbar*s Voltage17 kV Detection 254 nm, indirect 1 chloride 2 nitrite 3 sulfate 4 nitrate 5 fluoride 6 phosphate * bromide hollow ** hydrocarbonate

  13. 2.8 4 3 2 1 min 2 4 6 8 10 12 14 ANALYSIS OF PESTICIDES OF SYMMETRIC TRIAZINE GROUP Buffer10 mmol sodium borate, 32 mmol SDS Sampletest solution, 2.5 mg/l of each compound Capillaryl/L 60/70 cm, id 75 µm Injection900 mbar*s Voltage+20 kV Detection229 nm 1–simazine 2– atrazine 3– propazine 4– prometryne

  14. 9 8 14 4 10 2 3 11 7 5 13 1 6 12 10 20 min ANALYSIS OF SYNTHETIC DYES Buffer5 mmol phosphate, 5 mmol hydrocarbonate, pH 10.5 Samplestandard mixture of dyes Capillaryl/L 50/60 cm, id 75 µm Injection600 mbar*s Voltage+20 kV Detection254 nm 1– Rhodamine B (C.I. 45170) 2– Patent Blue V (E131, C.I. 42051) 3– Orange I (C.I. 14600) 4– Acid Green S (E142, C.I. 44090) 5– Acid Violet 5BN (C.I. 42650) 6– Quinoline Yellow (E104, C.I. 47005) 7– Sunset Yellow FCF (E110, C.I. 15985) 8– Tartrazine (E102, C.I. 19140) 9– Azorubine (E122, C.I. 14720) 10– Amaranth (E123, C.I. 16185) 11– Chrysoine (C.I. 14270) 12– Brilliant black PN (E151, C.I. 28440) 13– Indigotin (E132, C.I. 73015) 14– Brilliant Blue FCF (E133, C.I. 42090)

  15. ANALYSIS OF BENZOIC ACID DERIVATIVES Buffer25 mM borate pH 9.18 Capillary 26 / 35 cm, 50µm ID Injection 150 mbar x sec Voltage25 kV

  16. APPLICATIONS • Determination of inorganic anions in water samples • Determination of inorganic cations in water samples • Determination of phenoxycarboxylic acid herbicides in water • Determination of caffeine and preservatives in beverages

  17. SPECIFICATION

  18. CONTACTS LUMEX INTERNATIONAL 4736 Lee Hwy, suite 203, Arlington VA 22207, USA Phone/Fax: +1 (703) 243-7103; E-mail: info@lumexint.com; http://www.lumexint.com OhioLumex Co. 9263, Ravenna Rd., Unit A3 Twinsburg, OH 44087 USA Phone: +1 (888) 876-2611; +1 (330) 405-0837 Fax: +1 (330) 405-0847 E-mail: Siperst@yahoo.com, mail@ohiolumex.com; http://www.ohiolumex.com LUMEX LTD. 19, Moskovskiy pr., St. Petersburg, 190005 Russia Phone: +7 (812) 718-5390, +7 (812) 718-5391 Fax: +7 (812) 316-6538 E-mail: sales@lumex.ru, is@lumex.ru; http://www.lumex.ru

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