thể nghiệm oxidase xác nhận vi khuẩn sinh cytochrome c oxidase
Su01a1 lu01b0u1ee3c vu1ec1 thu1eed nghiu1ec7m Oxidase thu00ed u0111iu1ec3m oxidase u0111u01b0u1ee3c tiu00eau thu1ee5 u0111u1ec3 xu00e1c u0111u1ecbnh vi khuu1ea9n sinh cytochrome c oxidase, mu1ed9t enzyme cu1ee7a chuu1ed7i vu1eadn chuyu1ec3n u0111iu1ec7n tu1eed vi khuu1ea9n. khi hiu1ec7n diu1ec7n, cytochrome c oxidase oxy hu00f3a thuu1ed1c thu1eed (tetramethyl-p-phenylenediamine) thu00e0nh (indophenol) su1ea3n phu1ea9m cu00f3 mu00e0u tu00edm. khi khu00f4ng cu00f3 enzyme, thuu1ed1c thu1eed bu1ecb khu1eed vu00e0 khu00f4ng mu00e0u. Lu01b0u u00fd: mu1ecdi vi khuu1ea9n du01b0u01a1ng tu00ednh vu1edbi oxidase lu00e0 hiu1ebfu khu00ed, vu00e0 Cu00f3 thu1ec3 su1eed du1ee5ng oxy nhu01b0 1 chu1ea5t nhu1eadn u0111iu1ec7n tu1eed cuu1ed1i trong hu00f4 hu1ea5p. u0110iu1ec1u nu00e0y ko cu00f3 nghu0129a chu00fang lu00e0 cu00e1c VSV hiu1ebfu khu00ed cu1ea9n mu1eadt. Vi khuu1ea9n cu00f3 oxidase u00e2m tu00ednh Cu00f3 thu1ec3 lu00e0 ku1ef5 khu00ed, hiu1ebfu khu00ed, hou1eb7c phu00e2n lou1ea1i tu00f9y u00fd; Ku1ebft quu1ea3 u00e2m tu00ednh vu1edbi oxidase cu00f3 tu1ee9c lu00e0 nhu1eefng sinh vu1eadt nu00e0y khu00f4ng cu00f3 cytochrome c oxidase u0111u1ec3 u00f4xi hu00f3a thuu1ed1c thu1eed. Chu00fangcu00f3 thu1ec3 hu00f4 hu1ea5p bu1eb1ng phu01b0u01a1ng phu00e1p su1eed du1ee5ng cu00e1c oxidase khu00e1c trong di chuyu1ec3n u0111iu1ec7n tu1eed. Yu00eau cu1ea7u thu00ed u0111iu1ec3m u0111u1ed1i vu1edbi test Oxidase: Giu1ea5y lu1ecdc tu1ea9m vu1edbi cu01a1 chu1ea5t (1% tetramethyl-p-phenylenediamine dihydrochloride), hou1eb7c u0111u0129a giu1ea5y thu01b0u01a1ng mu1ea1i, que cu1ea5y gu1ed7 hou1eb7c que cu1ea5y bu1ea1ch kim. Ku1ebft quu1ea3 cu1ee7a thu00ed nghiu1ec7m Oxidase Du01b0u01a1ng tu00ednh: Xuu1ea5t hiu1ec7n mu00e0u tu00edm u0111u1eadm (indophenols) trong khou1ea3ng 10 giu00e2y u00c2m tu00ednh: khu00f4ng cu00f3 mu00e0u quy tru00ecnh thu1eed nghiu1ec7m Oxidase: Lu1ea5y giu1ea5y lu1ecdc u0111u00e3 ngu00e2m vu1edbi chu1ea5t nu1ec1n dihydrochloride tetramethyl-p-phenylenediamine Lu00e0m u1ea9m giu1ea5y bu1eb1ng nu01b0u1edbc cu1ea5t khu1eed tru00f9ng Chu1ecdn khuu1ea9n lu1ea1c u0111u1ec3 u0111u01b0u1ee3c thu1eed nghiu1ec7m bu1eb1ng que cu1ea5y gu1ed7 hou1eb7c bu1ea1ch kim vu00e0 bu00f4i trong giu1ea5y lu1ecdc Quan su00e1t vu00f9ng giu1ea5y u0111u01b0u1ee3c cu1ea5y xem su1ef1 biu1ebfn u0111u1ed5i mu00e0u thu00e0nh xanh u0111u1eadm hou1eb7c tu00edm u0111u1ed9 khou1ea3ng 10-30 giu00e2y buu1ed9c phu1ea3i thu1eadn tru1ecdng trong khi thu1ef1c hiu1ec7n xu00e9t nghiu1ec7m oxidase: khu00f4ng su1eed du1ee5ng que cu1ea5y hu1ee3p kim niken-base cu00f3 chu1ee9a crom vu00e0 su1eaft (nichrome) u0111u1ec3 lu1ea5y khuu1ea9n lu1ea1c vu00e0 bu00f4i khuu1ea9n ...
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