QIAgility ─ Pure Precision Andrea Tesoriero Application Specialist QIAGEN 2009

1. QIAgility ─ Pure Precision Andrea Tesoriero Application Specialist QIAGEN 2009

2. You Have More Important Things To Do…. 2007 2009 First we automated manual spin column preps… Now we are able to automate all manual pipetting in the lab… Now QIAGEN takes the next step and enables another convenience shift

3. From Manual to Automation Eliminating tedious pipetting in the lab! • Higher Precision and more time every day for… • Preparing & designing new experiments • Data analysis • Literature search • Writing scientific articles • And much more…

4. Developed For PCR SetupQIAgility Main Advantages PrecisionResults achieved exceed the best hand pipettingVersatilitySupports all different cycler formats in the marketEase-of-useWhat-you-see-is-what-you-get software interfaceSpeed Streamlined workflow by

5. QIAgility performs all “tricks” an experienced technician uses while pipetting Dynamic liquid level sensing limiting tip immersion Tracking the meniscus in each tube to minimize tip retention Retracting the tip while dispensing Minimizing Tip/Liquid contact Slowing down pipetting speed for viscous liquids Avoiding formation of bubbles in tip Selecting automatically which tip (50µl or 200µl) to select maximizing precision High Precision Meets Pipetting SkillsPrecision Aspects Precision

6. No matter what real-time instrument you use, QIAgility is compatible The system is pre-configured to work with all PCR tube types and all plate formats, including varieties of 96-well, 384-well and Rotor-Disc Beside any PCR setup the system can perform Set-up of quantification standards (dilution series) Normalization of sample concentration Sample pooling Transfer of samples from one tube format to another Automatic set up for multiple variables Post PCR setup The Hallmark Of QIAgility Is Its VersatilityVersatility Aspects Versatility

7. VersatilityQIAgility supports all real-time PCR formats 96-well and 384-well(Incl. all ABI, Bio-Rad, Stratagene, Eppendorf etc. instruments) SmartCycler™ Tubes Roche COBAS™ Rotor-Gene™ 0.2 mL, 0.1 mL and Gene-Disc™ formats LightCycler™ Capillaries

8. Versatility96 and 384-well support Compatible with plates, tubes, tube racks, cooling blocks etc

9. VersatilityUnusual formats are also supported Including: MALDI plates, ELISA plates, reservoir plates, etc, etc

10. The Intuitive Software Is Easy To UseSoftware Features Ease of Use The software mirrors the actual deck setup • Intuitive layout—simple to learn • Calculation of reagent needs according to the number of reactions and replicates specified • Tip re-use options • Import/Export of sample names • Pre- and Post-run reports including worktable setup guide • Virtual mode aids new protocol testing • Wizards for speedy PCR setup and optimization

11. You Have More Important Things To Do….QIAgility Means Time Savings Speed “If you worked once with the QIAgility, you don’t remember how you have managed without…” • Staff can be attending to other duties while the QIAgility is running • Simple Transfer Actions 1:1 • 96 samples • 17 minutes • 6 minutes with tip reuse • Transfer Actions with pre-MM • 96 samples PCR set-up • ~25 minutes • Transfer Actions with pre-MM • 192 samples PCR set-up • ~46 minutes

12. Unrivaled Pipetting PrecisionRotorGene Probe RT PCR Kit Precision Data presented in brochure table 1

13. Exceptional Reproducibility and Accurate ResultsRotorGene Probe PCR – Template dilutions Precision QIAgility • Setup Details • Target: IL1 R2 • Sample: Hs gDNA • 10 twofold dilutions (30ng – 60pg) • 5 replicates • Rotorgene 6000 Data presented in brochure figure 1

14. Automated Workflow for Accurate ResultsRotorGene Probe RT PCR Kit Precision Manual • Setup Details • Target: IL1 2RB1 • Sample: Leuko RNA • Standard row in triplicates • 5 tenfold dilutions (10 pg – 100 ng) • Rotorgene 6000 QIAgility Data presented in brochure fig 3

15. Worktable setup example Worktable Positions Master Mix Block Reagent Block Tips Tips Tips or Rotor-Gene Q Loading Block Tips Sample Plate or Rotor-Gene Q Loading Block Reaction Plate 3 4 1 5 6 2 7 8 3 4 1 6 5 2 7 8

16. Life Can Be So Simple….QIAgility PCR Setup Wizard Guides you through creating a run file with master mix, standard, Sample and final reaction!

17. Set Up Your Own RunSoftware Explanation Just a few simple steps • Define Plate/Tube Format • Sample Bank (number of samples) • Reagents (primer, buffer, taq, etc.) • Master Mix (pre-mix, setup) • Standards • Reactions • Add Controls • Add Standard • Add Samples • GO!

18. View the software