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UT SSV JLM JK MP

UT SSV JLM JK MP. Oprenset l DNA. Silica kugler. Buffer. 50 gr. bland hvert 2. min. Vask og tør kugler. Eluer ved lav saltkonc. Plasmid preperation fra positive kloner (hvide kolonier) skåret med Hin dIII. Linn Linn ASS ASS MI MI JKZ JKZ. pUC19 fragment.

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UT SSV JLM JK MP

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  1. UT SSV JLM JK MP Oprenset l DNA Silica kugler Buffer 50 gr. bland hvert 2. min Vask og tør kugler Eluer ved lav saltkonc.

  2. Plasmid preperation fra positive kloner (hvide kolonier) skåret med HindIII Linn Linn ASS ASS MI MI JKZ JKZ pUC19 fragment

  3. Plasmid preperation fra positive kloner (hvide kolonier) skåret med HindIII CD CD JV JV HKH HKH UT UT MP 10000 7000 5000 3000 2000 1500 1000 700 500 300 200 100 6 kb fragment pUC19 fragment

  4. Skæring af plasmidDNA med forskellige restriktionsenzymer EcoRi AatII EcoRI AatII SphI SmaI SphI EcoRI SacI Uskåret JLM JLM JLM JLM JK JK JK JK MI MI MI MI

  5. Skæring af plasmidDNA med forskellige restriktionsenzymer EcoRi SmaI AatII SphI BglI AatII SmaI AatII SmaI EcoRi SphI EcoRI SmaI MK MK MK MK MK JU1 JU2 JU3 DC1 DC2 DC3

  6. Skæring af plasmidDNA med forskellige restriktionsenzymer AatI1 Aat2 Sma1 Sma2 SmaI Sph1 EcoRI SmaI HindIII HKH HKH HKH HKH HKH UT1 UT2 ASS ASS ASS

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