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OABP Spring Meeting April 13 & 14, 2011 Guelph Ontario. Lignin Effects on Intake and Ruminal Digestion. Dr. Mike Brouk. mbrouk@ksu.edu 785-532-1207. Lignin – Enemy of Digestibility. Lower fiber digestibility Increased retention time in rumen Increased gut fill Lower intake
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OABP Spring Meeting April 13 & 14, 2011 Guelph Ontario Lignin Effects on Intake and Ruminal Digestion Dr. Mike Brouk mbrouk@ksu.edu 785-532-1207
Lignin – Enemy of Digestibility • Lower fiber digestibility • Increased retention time in rumen • Increased gut fill • Lower intake • Lower milk production • Reduced body condition score? • Reduced reproductive performance?
Van Soest Fiber Analysis Ground forage material Digest with neutral detergent solution Neutral Detergent Solubles ( Cell Contents ) Neutral Detergent Insoluble Fiber (NDF) Digest with acid detergent solution Acid Detergent Solubles Hemicellulose, cell wall N Acid Detergent Insoluble Fiber (ADF) Digest with sulfuric acid Solubles ( Cellulose ) Acid Insoluble Lignin
Digestibility • NDF Digestibility (NDFD) • 30 or 48 hours of incubation in rumen fluid • Estimation based on lignin content • in vitro procedure • 24, 30 or 48 hour
Corn Silage Composition and Maturity(Bal, et al. 1997. JDS 80:2497-2503)
Reduced Lignin and Animal Performance Oliver, et al. 2004
Lignin and Animal Performance Aydin, et al. 1999
Application of Lignin in Formulation • How does lignin impact fermentation? • What is the interaction between lignin and intake regulation?
Impact on Fermentation • Reduced digestibility • Increased retention time • Decreased intake Aydin, et al. 1999
Control of Feed Intake • Dry Cow – Transition • Mobilization of body fat - NEFA • Fermentability of the diet • Early to Peak Lactation • Distension • Post Peak • Fermentability of the diet • Plasma insulin concentration • Tissue sensitivity to insulin
Potential of Lignin to Influence Animal Performance • Early Lactation • Digestibility • Intake – Gut Fill • Post Peak • Digestibility
Challenges to Using Lignin in Formulation • Methods of testing • Within lab variation • Between lab variation Oliver, et al. 2004
Challenges to Using Lignin in Formulation • Grouping of animals • Single Lactation TMR’s • Lack of data on specific groups • Identification of first limiting factor of production or efficiency
Challenges to Using Lignin in Formulation • Source of lignin • Cottonseed hulls • By-product feeds • Lack of specific recommendations
Current Application in Formulation • Experimental • Limited in practical application due to on farm practices and measurements
Research Needs • Interaction of lignin with regulation of intake • Stages of production • Group specific performance data
Summary • Lignin can influence intake and digestibility • Lignin can influence productivity and efficiency of production • Limited recommendations for TMRs at this time • Variable results in the field • Many variables involved