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EXPERIMENT #4

EXPERIMENT #4. ACID FAST STAIN ENDOSPORE STAIN CAPSULE STAIN MOTILITY (Wet mount/Hanging drop). Acid Fast Stains. Primary Stain: Kinyoun Carbolfuchsin (w/ high concentration of phenol). - - - - - -. Non Acid Fast: GRAM +. 2. Acid alcohol: Decolorizer. Acid Fast: Mycobacterium.

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EXPERIMENT #4

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  1. EXPERIMENT #4 ACID FAST STAIN ENDOSPORE STAIN CAPSULE STAIN MOTILITY (Wet mount/Hanging drop)

  2. Acid Fast Stains

  3. Primary Stain: Kinyoun Carbolfuchsin • (w/ high concentration of phenol) - - - - - - Non Acid Fast: GRAM + 2. Acid alcohol: Decolorizer Acid Fast: Mycobacterium - - - - - -

  4. Primary Stain: Kinyoun Carbolfuchsin - - - - - - Non Acid Fast: GRAM + 2. Acid alcohol: Decolorizer Acid Fast: Mycobacterium - - - - - -

  5. Primary Stain: Kinyoun Carbolfuchsin - - - - - - Non Acid Fast: GRAM + 2. Acid alcohol: Decolorizer 3. Brilliant Green: Counterstain Acid Fast: Mycobacterium - - - - - -

  6. Primary Stain: Kinyoun Carbolfuchsin - - - - - - Non Acid Fast: GRAM + 2. Acid alcohol: Decolorizer 3. Brilliant Green: Counterstain Acid Fast: Mycobacterium - - - - - -

  7. KINYOUN ACID FAST STAIN

  8. 5 d 5 day old 5 days old) bacterial cultures Prepare a heat fix smear of the bacterial cultures

  9. ENDOSPORE STAIN • Primary stain: Malachite Green + HEAT • Decolorizer: Water • Counter stain: Safranin

  10. ENDOSPORE STAIN (Bacillus subtilis)

  11. 1. Congo Red ( - ) 2. Maneval's Stain (+)

  12. 1. Congo Red ( - ) 2. Maneval's Stain (+)

  13. Proteus vulgaris Proteus vulgaris Brownian motion vs True motion

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