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DNA Electrophoresis in Forensic Biology: Basic Principles and Applications

Learn the basic principles of DNA electrophoresis in forensic biology, including the separation of DNA fragments based on electric potential forces and mobility. Discover the matrices and apparatus used, such as agarose and polyacrylamide gels, for effective DNA analysis in forensics.

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DNA Electrophoresis in Forensic Biology: Basic Principles and Applications

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  1. Chapter 15: DNA Electrophoresis Forensic Biologyby Richard Li

  2. Basic Principles • Electrophoresis separates various sizes of DNA fragments • Based on migration of charged macromolecules in an electric field • Electrical Potential- force responsible for moving the charged macromolecule • Electrophoretic Mobility- determined by charge-to-mass ratio and shape • Relative mobility Forensic Biology by Richard Li

  3. Matrices • Physical support material • Agarose • Polyacrylamide • Polymerization Reaction • Cross-linking Reaction Forensic Biology by Richard Li

  4. Apparatus • Agarose • Slab Gel • Polyacrylamide • Slab Gel • Capillary Electrophoretic Apparatus Forensic Biology by Richard Li

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