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Varian 940-LC Analytical to Prep HPLC

Varian 940-LC Analytical to Prep HPLC. Varian 940-LC Silver Bullets. The 940-LC one instrument – many uses: High performance automated Analytical HPLC High performance semi-preparative HPLC High performance prep LC. Varian 940-LC Silver Bullets. Flexible and capable

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Varian 940-LC Analytical to Prep HPLC

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  1. Varian 940-LC Analytical to Prep HPLC

  2. Varian 940-LC Silver Bullets • The 940-LC one instrument – many uses: • High performance automated Analytical HPLC • High performance semi-preparative HPLC • High performance prep LC

  3. Varian 940-LC Silver Bullets • Flexible and capable • Wide flow rate from 0.1 ml/min to 200 ml/min • Dual path-length flowcells for extended Abs • One Injector, analytical or prep volumes (5ul to 4.5ml) • Superb fraction collecting • Galaxie real-time peak detection • Practical collect and inject • Much Easier interface, it does what you tell it! • Cleaner UI

  4. Goals of the 940-LC Analytical to Prep • Aim • Become market leader in this $180M segment • Double market share • Market • Continue to focus on Pharma / BioPharma • Academic • Strategy • Deliver innovation in hardware, consumables, software and services

  5. Varian 940-LC Instrument Overview • Factory tested, pre-plumbed instrument: • Plug and play operation • High performance semi-preparative HPLC • Gradient Analytical HPLC • Autoprep System • Choice of UV or PDA detector • Dual path flow cell for unmatched range and scalability • No compromise performance for prep and analytical – switch with a key stroke – no plumbing or hardware changes needed! • The most flexible analytical to prep HPLC available

  6. Varian 940-LC Instrument Overview • Scale-up Module for automated methods transfer from analytical to semi-prep, or switching from Analytical to Prep work • Versatile and robust solvent-delivery system • New loopless autosampler – wide volume range • NEW Fraction collection with • Complete range of collection modes such as slope, threshold and time slicing • Wide range of vessels, including autosampler rack • Comprehensive range of columns and consumables • More productivity via GalaxieTM software • Valve automation for genuine unattended operation • NEW direct control interface

  7. 1 ml/min 21 ml/min Varian 940-LC Versatility and Performance • Easy to change pump heads • Versatile solvent delivery systems • Powerful pumps allow 4000psi at 25mL/min operation for fast chromatography • Ideal for 4.6mm and 1” column • Able to use smaller 3 & 5 um particles columns at elevated pressures • Improves productivity • Save solvents and waste costs • Short run times save time • High resolution separations = high purity

  8. Varian 940-LC Pumps • Same range of accessories as PS210/218 • 5ml Head to 200ml Head • Easily interchangeable pressure modules thru removable side panel. • Easy access to mixer • Self maintenance and versatility features have been completely retained with no compromises – integrated systems are notoriously difficult to maintain so this is Unique

  9. Varian 940-LC Pumps - FAB

  10. Varian 940-LC Autosampler • Prep version of the 920-LC Sampler • 5ul to 4.5ml Injection range • No necessity to combine a Manual injector • No need to dedicate injector to prep or analytical • One Sampler does all! • Cooled version • Loopless Design • Needle in path Design • No carryover • 2008 Technology – no changing loops

  11. Varian 940-LC Autosampler - FAB

  12. Varian 940-LC UV/Vis - PDA Detectors • High Sensitivity for Analytical applications • Extended absorbance – desensitised, for Prep Applications • All in One Cell! • NEW 4 x 0.25mm Cell low dead volume • Excellent performance at low flow rates for Analytical • Excellent Extended Absorbance for Prep

  13. Varian 940-LC Dual Pathlength Flowcell • Absorbance Detector • Extended absorbance range without re-plumbing (<70 AU) • NEW 4mm x 0.25 mm • 4mm x 0.15 SuperPrep • Low dispersion • UV/Vis Detector • PDA Detector • Low noise ~ +10 mAU • Low Drift ~ 1 mAU/hr

  14. Agilent 1200 Flowcells

  15. Varian 940-LC UV/Vis - PDA Detectors - FAB

  16. Varian 440-LC Fraction Collector • Fully controlled by software for foolproof collection by slope, level or timeslice • Known fluid path for accurate fraction collection • Software display of collected fractions and source injections • Reliable operation • Compatible with a wide range of containers • Belart and other optional racks • Microvial to 50ml tube capacity • Autosampler rack compatibility provides practical collect and re-inject capabilities

  17. Varian 440-LC Fraction Collector • We chose not to collect Peak 3 • Peaks 1,2,4,5,6,7 collect with exceptional accuracy • Excellent algorithm, and simple delay volume calculation • Slope/Threshold based on real numbers!!

  18. Varian 440-LC Fraction Collector • Fraction Log shows peak location using colour coded rack assignment

  19. Varian 440-LC Fraction Collector - FAB

  20. Optimized flow paths for automated scale-up Separate optimised flow paths for Prep and Analytical columns Solvent selection valves for 4 solvents ( 2 per pump) Integrated priming valves for easy solvent changes Two Integrated Push Button Manual valves for Analytical and prep Injections Varian 445-LC Scale-up Module

  21. Varian 445-LC Scale Up Module( SUM) - FAB

  22. Varian 940-LC Software Usability • Software designed for Prep users • Enhanced manual control • One page status and chromatogram • Real time fraction collection display • Advanced peak sensing for better fraction collection • Purity displays for absorbance detection – standard! • Documentation and audit trails for traceability and regulatory needs

  23. Varian 940-LC Configurations

  24. Varian 940-LC Consumables • Columns designed for Prep users • From 3 to 10mm media • Wide range of C4 to C18 • Silica and Polymer media • From 2mm to 2” ID • Unique solutions for dedicated prep with load and lock systems • Matched Analytical to Prep columns and packing's to assure smooth scale up • Solutions for hardware, software and consumables

  25. Varian 940-LC Summary The 940-LC one instrument – many uses • High performance automated Analytical HPLC • High performance semi-preparative HPLC • Versatility • Pumping system able to operate at analytical flow rates and scalable up to prep flows • Dual path-length flow cell technology for unmatched range • Scale-up Module for automated methods transfer • Advanced fraction collection with full software control • Complete range of columns and consumables • Prep enhanced Galaxie TM software and valve automation for genuine unattended operation

  26. Conclusion for 900 Series Compact and Stylish Integrated Systems Retain flexibility and maintenance features Top Tier hardware performance Top Tier User Interface – pleasant experience Simplicity in a box – Install , Use and Maintain 1st Truly Integrated HPLC’s on the Market 26

  27. Questions??

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