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Supplemental figure S1

Exon 1. a. Bam HI. Eco R1. Xba I. Bam HI. i. MYH9 locus. probe. 6.6 kb. in vivo recombination. Bam HI. Bam HI. Eco R1. Bam HI. NEO. ii. Targeted allele. 8.3 kb. in vitro cre recombination. MYH9 Flx. iii. in vivo cre recombination. MYH9 Δ. iv. b. Southern Blot. c. PCR.

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Supplemental figure S1

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  1. Exon 1 a BamHI EcoR1 XbaI BamHI i MYH9 locus probe 6.6 kb in vivo recombination BamHI BamHI EcoR1 BamHI NEO ii Targeted allele 8.3 kb in vitro cre recombination MYH9Flx iii in vivo cre recombination MYH9Δ iv b. Southern Blot c. PCR +/+ +/- -/- ES +/+ ES +/- 8.3 kb 6.6 kb MYH9Flx wt Supplemental figure S1

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