1 / 30

Protein Traffic Roadmap: Sorting Signals and Intracellular Transport

Explore the intricate pathways of protein trafficking within the cell, including the two ways in which sorting signals can be built into a protein. Learn about nuclear pore complexes, nuclear import signals, transmembrane transport, protein import into mitochondria and chloroplasts, protein transport into peroxisomes, and the role of protein glycosylation in the ER. Discover how these processes ensure proper protein localization and folding for cellular functions.

garydavis
Download Presentation

Protein Traffic Roadmap: Sorting Signals and Intracellular Transport

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Roadmap of protein traffic inside cell

  2. Two ways in which a sorting signal can bebuilt into a protein

  3. Some typical signal sequences

  4. Nuclear Pore complexes perforate the nuclear envelope

  5. Nuclear pore complexes

  6. Nuclear side of the nuclear envelope

  7. Face on view of nuclear complexes without the membrane

  8. Side view of 2 nuclear pore complexes

  9. Transport through nuclear pore complexes occurs through free diffusion and active transport

  10. Nuclear import signal direst proteins to the nucleus

  11. Single amino acid mutation in signal will prevent import into the nucleus

  12. Nuclear import receptors bind to nuclear porins and nuclear localization signal of cargo protein Different nuclear localization signals bind different import receptors

  13. Ran GTP provides energy for nuclear protein import

  14. Ran-GTP controls cargo loading and unloading

  15. Transmembrane transport into the mitochondria and chloroplasts

  16. Subcompartments of mitochondria and chloroplasts

  17. Signal sequence for mitochondrial import red = positively charged yellow = nonpolar

  18. Signal sequence for mitochondrial import can form amphipathic α - helix α - helix is recognized by receptor proteins

  19. Protein translocators in mitochondrial membrane

  20. Protein import by mitochondria

  21. DVD Clip 55

  22. Protein transport into the peroxisomes

  23. Roadmap of protein traffic inside cell

  24. Insertion of rhodopsin into the ER membrane

  25. Most proteins in the ER are glycosylated Proteins in cytosol are rarely glycosylated original precursor oligosaccharide added to most proteins in the ER

  26. Protein glycosylation in the rough ER

  27. Oligosaccharides are used as tags to mark the state of protein folding

  28. Misfolded proteins are exported from ER and destroyed

More Related