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Microscopy

Microscopy. Tools of the Trade. Microscopy Skills. Field Of View Low Power High Power Measurement: 1500 um Measurement: 375 um Magnification: 100X Magnification: 430X. Microscopy Skills. Part # 1 Eye Piece

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Microscopy

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  1. Microscopy

  2. Tools of the Trade

  3. Microscopy Skills Field Of View Low Power High Power Measurement: 1500 um Measurement: 375 um Magnification: 100X Magnification: 430X

  4. Microscopy Skills

  5. Part # 1 • Eye Piece • Lens that magnifies 10X – What you look through.

  6. Part # 2 • Course Adjustment • The inner knob that allows you to move the eye piece and focus

  7. Part # 3 • Fine Adjustment • Fine tunes your focus – Never move this when in high power.

  8. Part # 4 • Oil Immersion Lens • You do not need to know, but it is a lens on your microscope

  9. Part # 5 • Arm • Used to carry the microscope and bend the microscope back

  10. Part # 6 • Hinge • Allows you to bend the microscope back

  11. Part #7 • Body Tube • Connects the eye piece to the objectives

  12. Part #8 • Revolving Nose Piece • 2-3 objectives and allow them to rotate

  13. Part # 9 • High Power Objective • 40X or 43X magnification…you see things 400 or 430 times larger

  14. Part # 10 • Low Power Objective • 10X magnification… • you see things 100 times large

  15. Part # 11 • Stage Clips • Holds slides in place

  16. Part # 12 • Stage • Supports the slide and allows light through

  17. Part # 13 • Diaphragm • Controls the amount of light that goes through the stage

  18. Part # 14 • Light* • or Mirror • Directs light through specimen in order to see it

  19. Part # 15 • Base • Supports the entire microscope

  20. Eye piece Course adjustment Fine adjustment Oil Immersion lens Arm Hinge Body tube Nose piece High power objective Lower power objective Stage clips Stage Diaphragm Light* / mirror Base

  21. Preparing a Wet Mount Slide • Using a water dropper, place 1 drop of water on the center of a clean slide. • With a pare of forceps, place the sample onto the drop of water. • Carefully take a clean coverslip and place on the edge of the drop. Let the cover fall gently on the drop.

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