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Microscopy

By Jane Horlings. Microscopy. Microscopy. Robert Hooke, circa 1700s. Electron Microscope, late 1900s. Light Microscopes. Early light microscope (UL), drawing by Hooke (LL). Light Microscopes. Dissecting (stereo) microscope (L), compound microscope (R). Light Microscopy; Plant Cells.

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Microscopy

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  1. By Jane Horlings Microscopy

  2. Microscopy Robert Hooke, circa 1700s Electron Microscope, late 1900s

  3. Light Microscopes Early light microscope (UL), drawing by Hooke (LL)

  4. Light Microscopes Dissecting (stereo) microscope (L), compound microscope (R)

  5. Light Microscopy; Plant Cells Onion root tip, cell division (L), shoot tip (R)

  6. Light Microscopy, Phase Contrast Single celled Amoeba dividing (L), green alga Micrasterias (R)

  7. Light Microscopy Fluorescence Confocal Phase contrast

  8. Electron Microscopes

  9. Limitations of Electron Microscopy • Works in a vacuum • Specimens are dead, chemically preserved; no life processes can be seen • No color (colorized by artist on computer)

  10. Electron Microscopes Transmission Electron Microscope (TEM) Scanning Electron Microscope (SEM)

  11. Scanning Electron Microscopy TEM of cilia SEM of cilia

  12. Bacterium SEM RBCs in clot

  13. SEM Mite (UL) Gecko toes (LL) Shark skin (LR)

  14. SEM Trypanosome Red blood cells Red blood cells and Trypanosoma (L), Giardia (R)

  15. Transmission Electron Microscopy TEM of cilia SEM of cilia

  16. TEM Viruses (L), animal cell (R)

  17. TEM Animal cell (L), muscle tissue (R)

  18. Light Microscopy • Based on light • Specimens can be alive; life processes can be seen • Color; dyes may be used

  19. Parts of the Microscope • Ocular lenses • Objective lenses • How to compute the magnification

  20. Use of the Microscope • Place slide in center • Adjust light, lenses, barrel • Put on low magnification! • Move stage all the way up and then back down half a turn!

  21. Use of the Microscope • Look and readjust focus • Move to higher magnification if needed

  22. Use of the Microscope • Importance of focusing with the fine adjustment! • Oil immersion lens • How to adjust the light

  23. End

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