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PARAMETERS. NQAD GAS: Air FILTER: 1.3 sec GAIN: 1X EVAPORATION TEMP: 35C ELSD GAS: Nitrogen, 1.5 L/min GAIN: 1 EVAPORATION TEMP: 40C HPLC MOBILE PHASE: 30.70 ACN:Water with 0.2% TFA. APPLICATION NOTES. FLOW RATE: 1.00 mL/min COLUMN: Phenomenex Kinetex C18, 150 x 4.6, 2.6 u
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PARAMETERS NQAD GAS: Air FILTER: 1.3 sec GAIN: 1X EVAPORATION TEMP: 35C ELSD GAS: Nitrogen, 1.5 L/min GAIN: 1 EVAPORATION TEMP: 40C HPLC MOBILE PHASE: 30.70 ACN:Water with 0.2% TFA APPLICATION NOTES FLOW RATE: 1.00 mL/min COLUMN: Phenomenex Kinetex C18, 150 x 4.6, 2.6 u COLUMN TEMP: Ambient INJECTION VOLUME :1 µL ANALYTE: Tetracycline ™ QUANT TECHNOLOGIES, LLC 1463 94TH Lane NE Blaine, MN, USA 55449 763-398-0508 www.Quant-NQAD.com THE NQAD IS HIGHLY LINEAR, EVEN AT LOW CONCENTRATIONS WHICH ARE UNDETECTABLE BY THE ELSD Application note # QAN-0090 CHROMATOGRAMS Note: This application was run at Quant Technologies on a single ELSD and may not be representative of all ELSD’s.