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THE NQAD IS HIGHLY SENSITIVE, ALLOWING MORE SAMPLE COMPONENTS TO BE DETECTED

PARAMETERS. 9.00. mV. NQAD GAS: Air FILTER: 1.3 sec GAIN: 1X EVAPORATION TEMP: 35C ELSD GAS: Nitrogen, 1.5 L/min GAIN: 1 EVAPORATION TEMP: 40C HPLC MOBILE PHASE: 45:55 water:ACN. min. 4.00. 0.00. 0.20. 0.40. 0.60. 0.80. 1.00. 1.20. 1.40. 1.60. 1.80. 2.00. 2.20. 2.40.

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THE NQAD IS HIGHLY SENSITIVE, ALLOWING MORE SAMPLE COMPONENTS TO BE DETECTED

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  1. PARAMETERS 9.00 mV NQAD GAS: Air FILTER: 1.3 sec GAIN: 1X EVAPORATION TEMP: 35C ELSD GAS: Nitrogen, 1.5 L/min GAIN: 1 EVAPORATION TEMP: 40C HPLC MOBILE PHASE: 45:55 water:ACN min 4.00 0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 48.00 mV FLOW RATE: 1.0 mL/min COLUMN: Thermo BetaBasic C18, 150 x 4.6 mm, 5u COLUMN TEMP: Ambient INJECTION VOLUME :10 µL ANALYTE: Hydrocortisone QUANT TECHNOLOGIES, LLC 1463 94TH Lane NE Blaine, MN, USA 55449 763-398-0508 www.Quant-NQAD.com min 45.00 0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 THE NQAD IS HIGHLY SENSITIVE, ALLOWING MORE SAMPLE COMPONENTS TO BE DETECTED Application note # QAN-0058 CHROMATOGRAMS 1 NQAD, 10 ng Avg S/N, 53.5 APPLICATION NOTES 2 1 ELSD, 10 ng Avg S/N, 2.8 ™ 2 1 = Injection Artifact, 2 = Hydrocortisone, 10 ng

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