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THE NQAD IS HIGHLY REPRODUCIBLE, EVEN AT LOW CONCENTRATIONS

CHROMATOGRAMS. NQAD GAS: Air FILTER: 1.3 sec GAIN: 1X EVAPORATION TEMP: 35C ELSD GAS: Nitrogen, 1.5 L/min GAIN: 1 EVAPORATION TEMP: 40C HPLC MOBILE PHASE: 20:80 ACN:Water. NQAD S/N = 100.3 % RSD = 1.37 (n = 5). 46.40. mV. 5.200. mV. FLOW RATE: 1.0 mL/min

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THE NQAD IS HIGHLY REPRODUCIBLE, EVEN AT LOW CONCENTRATIONS

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  1. CHROMATOGRAMS NQAD GAS: Air FILTER: 1.3 sec GAIN: 1X EVAPORATION TEMP: 35C ELSD GAS: Nitrogen, 1.5 L/min GAIN: 1 EVAPORATION TEMP: 40C HPLC MOBILE PHASE: 20:80 ACN:Water NQAD S/N = 100.3 % RSD = 1.37 (n = 5) 46.40 mV 5.200 mV FLOW RATE: 1.0 mL/min COLUMN: Thermo BetaBasic C18, 150 x 4.6 mm, 5u COLUMN TEMP: Ambient INJECTION VOLUME :10 µL CONCENTRATION: 80 ng on column ANALYTE: Caffeine min ELSD S/N = 43.6 % RSD = 10.40 (n = 5) 0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 QUANT TECHNOLOGIES, LLC 1463 94TH Lane NE Blaine, MN, USA 55449 763-398-0508 www.Quant-NQAD.com min 4.400 0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 Note: This application was run at Quant Technologies on a single ELSD and may not be representative of all ELSD’s. THE NQAD IS HIGHLY REPRODUCIBLE, EVEN AT LOW CONCENTRATIONS Application note # QAN-0082 PARAMETERS APPLICATION NOTES ™

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