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Urinalysis Tidbits

Urinalysis Tidbits. Stains. Sternheimer-Malbin Crystal Violet and Safranin O Lipid Stains Oil Red O and Sudan III Stain Trigs and Neutral Fat but Not Cholesterol Cholesterol polarizes Cholesterol Drops Maltese Cross Cholesterol Crystals Birefringence. Microscopic Sediment Prep.

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Urinalysis Tidbits

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  1. Urinalysis Tidbits

  2. Stains • Sternheimer-Malbin Crystal Violet and SafraninO • Lipid Stains • Oil Red O and Sudan III • Stain Trigs and Neutral Fat but Not CholesterolCholesterol polarizes • Cholesterol Drops Maltese Cross • Cholesterol Crystals Birefringence

  3. Microscopic Sediment Prep • 12 mL • Spin 5 min at 400 RCF • RCF Relative Centrifugal Force • RCF = 1.118 x 10-5 x radius in cm x RPM2 • Examine a Min of 10 Fields Low and High

  4. Microscopy • Bright field • Polarized • Phase Contrast • Interference

  5. Red Blood Cells • Pale red • Ghost Cells Dilute or Alkaline Lyse • Hypertonic Conditions Crenate • 2% Acetic Acid Lyse • Yeast and Calcium Oxalate do not disappear with 2 % acidic acid

  6. WBC • Granular and often visible nucleus • Hypertonic will shrink • Hypotonic “Glitter” cells associated with Infection and Irritation & Pyelonephritis • Glitter cells show Brownian movement

  7. Epithelial Cells • Squamous Contamination • Transitional Bladder • Renal Tubular Renal Tubules

  8. Squamous Cells • Large, flat irregular shape • Small nucleus • Urethra and contamination • Not considered Clinically Sig

  9. Clue Cell • Vaginal Infection w Gardnerella vaginalis • Squamous cell covered w coccobacillus • Covers most of cell and extends beyond edges • Vaginal wet prep but may appear in urine

  10. Renal Tubular Cell • Slightly larger than WBC • Eccentric nucleus • Increased numbers Tubular damage • Pyelonephritis, Acute Tubular necrosis

  11. Hemosiderin • Not birefringent unlike urates or phosphates • Prussian blue dye stains iron of Hb blue

  12. Transitional Cells • Smaller than Squamous • Several Forms Caudate, spherical and polyhedral • Central nucleus • Renal Pelvis, calyses, ureters and bladder • Not Clinical Sig catheterization

  13. Starch granules • Source Powdered Gloves • Maltese cross Polarized light • Look like a pouch

  14. Crab • Pediculosis pubis

  15. Pinworm • Enterobiusvermicularis • Ova one side flat • Adult male

  16. Schistosoma hematobium • World wide problem Egypt, Iraq, Syria, Iran Africa • US lacks the snail intermediate host • Trematode Terminal spine

  17. Triple Phosphate • Tri Phosphate going into solution

  18. RTE

  19. Mite • Adult mite • Scabies • Smaller than crab

  20. UA Web Sites • Comprehensive Review • http://www.aafp.org/afp/2005/0315/p1153.html

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