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CM-Toyopearl. Enzyme activity (U/ml). - - - NaCl (mM). Butyl-Toyopearl. Enzyme activity (U/ml). - - - (NH 4 ) 2 SO 4 (% saturation). Toyopearl HW-55. Enzyme activity (U/ml). Enzyme activity (U/ml). Fraction number. Supplementary Figures. Orikasa and Oda
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CM-Toyopearl Enzyme activity (U/ml) - - - NaCl (mM) Butyl-Toyopearl Enzyme activity (U/ml) - - - (NH4)2SO4 (% saturation) Toyopearl HW-55 Enzyme activity (U/ml) Enzyme activity (U/ml) Fraction number Supplementary Figures. Orikasaand Oda Elution profiles of three chromatographies of ion-exhange (CM-Toyopearl), hydrophobic interaction (Butyl-Toyopearl) and gel filtration (Toyopearl HW-55).