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Microscopes & You. Microscopes in Depth Fall 2017. Microscopes. What we use!. Magnifies organisms Types: Light Microscopes Electron Microscopes MUCH higher magnification Most powerful! Scanning (SEM) & Transmission (TEM) Magnification – increase in apparent size
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Microscopes & You Microscopes in Depth Fall 2017
Microscopes What we use! • Magnifies organisms • Types: • Light Microscopes • Electron Microscopes • MUCH higher magnification • Most powerful! • Scanning (SEM) & Transmission (TEM) • Magnification – increase in apparent size • Resolution –helps with clarity
Diaphragm Base Always carry a microscope with one hand holding the arm and one hand under the base. Ocular lens(Eyepiece) Body Tube Nosepiece Arm Objectives Stage Stage Clips Coarse Adjustment Fine Adjustment Light
CHECK FOR UNDERSTANDING! • What knob would you use to enhance magnification? • What knob would you use to enhance resolution? COARSE ADJUSTMENT KNOB FINE ADJUSTMENT KNOB
Comparing Powers of Magnification • We can see better details with higher the powers of magnification, but we cannot see as much of the image. • Which of these images would be viewed at a higher power of magnification?
Calculating Magnification • Eyepiece = 10x • Objective • Scanning = 4x (shortest in length) • Low Power = 10x • High Power = 40x (longest) • Magnification = EyepiecexObjective • Field of view measured in microns KNOW PARTS OF MICROSCOPE AND WHAT THEY DO!!
40x 100x 400x