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Engineering E.Coli to see Light

Engineering E.Coli to see Light. Voigt CA et. al. 2005. Light Engineering Imaging using E.Coli. Stimulus. Output. System. Sensor. Signal Transducer. Effector. Stimulus. Red Light Highly defined edges Two-dimensional image. Extracellular sensor. Intracellular regulator. Sensor.

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Engineering E.Coli to see Light

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  1. Engineering E.Coli to see Light Voigt CA et. al. 2005

  2. Light Engineering Imaging using E.Coli

  3. Stimulus Output System Sensor Signal Transducer Effector

  4. Stimulus • Red Light • Highly defined edges • Two-dimensional image

  5. Extracellular sensor Intracellular regulator Sensor • Phytochrome • Membrane bound extracellular sensor • Intracellular regulator • Fusion protein: phytochrome and EnvZ histidine kinase domain

  6. Photoreceptor Expression • PCB not produced in E.Coli • Introduction of PCB synthesis genes • Converts haem into PCB

  7. Transduction Mechanism • Red light represses EnvZ auto-phosphorylation

  8. Output • OmpR-P activates promoter linked to reporter gene • lacZ reporter produce black compound OmpR-P dependent ompC promoter

  9. System Revisited

  10. Review • Created novel genetic circuit • High definition film • (~100 megapixels per square inch) • lacZ expression as a function of light intensity / wavelength • Transfer function

  11. Something for the team to think about!!

  12. Light Canon

  13. Characterisation • Macro • Resolution • Micro • Transfer function • SigB activity as a function of: • Intensity • Wavelength

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