1 / 21

Laser Performance Testing in Instrument Field for Improved Resolution

Validate instrument performance through tests on field illumination, axial resolution, chromatic aberration, laser power, stability, and beam alignment. Deviation measurements and evaluations at UV field illumination intervals for various objectives and test samples.

hmatthew
Download Presentation

Laser Performance Testing in Instrument Field for Improved Resolution

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Instrument Performance --Tests • Field illumination (plastic slides, beads) • Axial resolution (mirror) • Chromatic aberration (1 u beads, mirror) • Laser power (meter, test sample) • Laser stability (plastic slide, transmission detector) • Beam alignment (focal check,mirror,bead)

  2. Deviation + 8%

  3. Deviation + 4%

  4. Deviation + 17%

  5. 647 488 568 Deviation = +10%, +17%, +13%

  6. UV Field Illumination 12.5% intervals 47 123 INTENSITY=230 100X Objective (1.4 NA)

  7. 5X Objective (.25 NA) 12.5% intervals

  8. 20X Objective (.7 NA) 12.5% intervals

  9. Axial Registration Mirror Test 63X objective (1.2NA) (Chromatic Aberration)

  10. 63x ( 1.2NA) objective 647 568 488

  11. 568 647 488

  12. Bad Galvanometer

  13. 488 nm + 568nm 568 nm 488 nm + 568nm 568 nm

  14. Test Samples • Beads: Spherotech 10 micron, MP focal check, 1 micron, submicron • Front silvered mirror • Silicon chip • Biological samples-Diatoms, Pollen, MP Fluo slide, User defined specimen • Fluorescence Plastic Slide

  15. A B C C F E D

  16. Dry Objectives • Lower NA’s --Less resolution • Longer working distance • Cleaner working environment

More Related