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pAg1-H3 (Zhang et al . 2003)

pAg1-H3 (Zhang et al . 2003). pPgpd-DsRed (Mikkelsen et al . 2003). PCR amplification of PgpdA. SmaI. pAg1-HU(LB). pAg1-H3E. LB UCS. LB UCS. ApaI/SacI. KpnI/XmaI. pAg1-HUE(LB). pRF-HU2. pRF-HU. pRF-HUE. pRF-HU2E. SpeI/HindIII. SmaI/HindIII. SmaI/HindIII. SpeI/HindIII. RB UCS.

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pAg1-H3 (Zhang et al . 2003)

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  1. pAg1-H3 (Zhang et al. 2003) pPgpd-DsRed (Mikkelsen et al. 2003) PCR amplification of PgpdA SmaI pAg1-HU(LB) pAg1-H3E LB UCS LB UCS ApaI/SacI KpnI/XmaI pAg1-HUE(LB) pRF-HU2 pRF-HU pRF-HUE pRF-HU2E SpeI/HindIII SmaI/HindIII SmaI/HindIII SpeI/HindIII RB UCS RB UCS (self-ligation) (self-ligation) For targeted gene replacement For general cloning purposes and testing of the system For overexpression of introduced genes from a random genome location For targeted overexpression of genes already found in the genome

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