Study and Monitoring of different contaminants in river water using an Immunosensor .

1. Lock In PD polymer fibres filter laser PC absorbing coating bevelled waveguide polished end-face inject FIA - System samples waste 1.00 1.25 1.25 1.00 1.00 1.00 1.00 0.75 g/L) g/L) m m m g/L) g/L) 0.75 0.75 0.75 0.75 ( ( µ µ 0.50 Biosensor Biosensor LC-MS 2,4 D (( 2,4 D ( 0.50 0.50 isoproturon isoproturon 0.50 0.50 (total triazines ( ) 0.25 0.25 0.25 0.25 0.25 0.00 0.00 0.00 0.25 0.25 0.50 0.50 0.75 0.75 1.00 1.00 0.00 0.25 0.50 0.75 1.00 1.25 1.50 0.00 0.00 0.25 0.25 0.50 0.50 0.75 0.75 1.00 1.00 certified certified 2,4 D 2,4 D sample sample ( ( µ µ g/L) g/L) (mg/L) sample certified isoproturon Biosensor (total triazines (mg/L)) • Study and Monitoring of different contaminants in river water using an Immunosensor. • SRodríguez-Mozaza, E, Mallata, R, Abukneshac, G. Gauglitzb and D. Barcelóa • aDept. of Environmental Chemistry, CID-CSIC, 08034 Barcelona • bInstitute of Physical Chemistry, University of Tübingen, 72076 Tübingen, Germany • cKing’s College London, University of London, W8 7AH London, England The immunosensor RIANA was applied to the trace determination and validation of different contamintants: atrazine, simazine, alachlor, isoproturon, 2,4-D and paraquat.The inmunosensor system detection mechanism is based on a solid-phase fluoroinmunoassay combined with an optical transducer chemically modified with an analyte derivativeThe results obtained with the immunosensor were correlated with classical chromatographic techniques such as, SPE-LC-MS. Objectives Application of the immunosensor to monitor organic contaminants in river water samples Study the effect of cross-reactants and the matrix on the ìmmunosensor response Confirmation of the results obtained by the immunosensor using conventional analytical methods Cross-reactivity IC50= Inhibition concentration at 50% of the absorbance Validation Encanyissada lagoon Results Detection limits in river water samples were around 0,1-0,2 μgL-1 Some overstimation of real values was observed owing to the presence of cross reactants and matrix effects. No false positives were detected. Validation of the immunosensor by SPE-LC-MS, GC-MS and EC-uv Acknowledgements This work has been supported by the comission of the European communities, RIANA (ENV-CT95-0066), and AWACSS (EVK1-CT-2000-00045) RIANA partners:Institute of Physical Chemistry, Univ. Tübingen, Germany; Optoelectronics Research Centre, Univ. Southampton, United Kingdom; Central Research Laboratories, Hayes Middlesex, Unitd Kingdom; DVGW- Technologiezentrum Wasser, Karlsruhe, Germany; Environmental Intstitute Kos, Slovak Republic; King’s college London, United Kingdom; Siemens, Erlangen, Germany; CID-CSIC, Barcelona, Spain; Major subcontractor: Catalan Water Agency (Agència Catalana de l’Aigua) Barcelona, Spain,