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Guidance of data collection for QVRA in WP5

Guidance of data collection for QVRA in WP5. Martijn Bouwknegt, Saskia Rutjes, Ana Maria de Roda Husman. Rinsing water. Animal manure. Quantitative Viral Risk Assessment (QVRA): soft fruits and vegetables. Toilet. Door-handle. Food Handlers. (Customers). Harvester’s hands.

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Guidance of data collection for QVRA in WP5

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  1. Guidance of data collection for QVRA in WP5 Martijn Bouwknegt, Saskia Rutjes, Ana Maria de Roda Husman

  2. Rinsing water Animal manure Quantitative Viral Risk Assessment (QVRA): soft fruits and vegetables Toilet Door-handle Food Handlers (Customers) Harvester’s hands Virus inactivation Virus inactivation Produce (production) Produce (processing) Produce (retail) Irrigation water Knives Virus inactivation Conveyor belt

  3. Selected products • Soft fruits: raspberries, strawberries • Salad vegetables: lettuce heads • Pork: pork sausage (raw/undercooked) • Shellfish: oysters, mussels?

  4. Guidance document on sampling • Need for individual guidance documents concluded during Novi Sad meeting → individual, location-specific guidance documents • Generation of guidance documents: • Sampling locations based on WP6 BRQ • Sampling points based on BRQ

  5. SP1 SP2 SP3 SP4 SP5

  6. Guidance document on sampling • Need for individual guidance documents concluded during Novi Sad meeting → individual, location-specific guidance documents • Generation of guidance documents: • Sampling locations based on WP6 BRQ • Sampling points based on BRQ • Most optimal distribution of samples between sampling points • Increasing probability of virus detection • Aim to most accurate estimate

  7. Number of samples for WP5 (QVRA) • Total sample sizes per DGL per production chain corrected with a reduction of 15% 212 212 212 45 For QVRA: 6 samples per phase for ad-hoc sampling

  8. Current status of the guidance docs

  9. Fresh produce: production Summary of all sampling points in the guidance documents * HAV: hepatitis A virus; NoV: norovirus; PAdV: porcine adenovirus; BoPV: bovine polyomavirus; HAdV: human adenovirus

  10. Fresh produce: processing Summary of all sampling points in the guidance documents * HAV: hepatitis A virus; NoV: norovirus; PAdV: porcine adenovirus; BoPV: bovine polyomavirus; HAdV: human adenovirus • No processing for: • Vegetables in Greece • Soft fruits in Czech Republic

  11. Fresh produce: point of sale (discussion) • Type of premises to be decided with WP6 (BRQ) • Supermarket • Farmers market • Number of visits • One location sampled frequently (e.g., once in two weeks) • Linked to production and processing • Type of produce • Fresh and frozen (soft fruits) • Imported vs. locally produced

  12. Pork

  13. Pork: selection of product (discussion) • Italy: meat sausage • UK: meat sausage • Spain: ? • Czech Republic: ?

  14. Pork: production (UK, IT) * HEV: hepatitis E virus; PAdV: porcine adenovirus

  15. Pork: processing * HEV: hepatitis E virus; PAdV: porcine adenovirus

  16. Pork: point of sale (discussion) • Type of premises to be decided with WP6 (BRQ) • Supermarket / butchershops • Link between phases not feasible • Number of visits • One store sampled frequently (e.g., once in two weeks) to sample sausage from different batches • Pork liver at retail or only sausages • PhD’s studies?

  17. Shellfish (Finland, Greece, Spain) Number of samples: 45 • Type of shellfish? • Types of production locations? • Locally grown vs. imported • Viruses to be detected

  18. Sufficient amount of RNA solution?

  19. Data reporting sheet (Excel) • Uniform coding of samples: • Absolutely necessary data for QVRA per sample: • Ct values of all controls • Ct values of target virus • Equivalent volume tested → the VITAL tool can be used for this

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