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A B C D E F. DNA Sample Key. A: DNA from crime scene with Enzyme 1 B: DNA from crime scene with Enzyme 2 C: DNA from suspect 1 with Enzyme 1 D: DNA from suspect 1 with Enzyme 2 E:DNA from suspect 2 with Enzyme 1 F: DNA from suspect 2 with Enzyme 2. Restriction Enzymes.

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A B C D E F

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  1. A B C D E F

  2. DNA Sample Key • A: DNA from crime scene with Enzyme 1 • B: DNA from crime scene with Enzyme 2 • C: DNA from suspect 1 with Enzyme 1 • D: DNA from suspect 1 with Enzyme 2 • E:DNA from suspect 2 with Enzyme 1 • F: DNA from suspect 2 with Enzyme 2

  3. Restriction Enzymes • Cut DNA in at specific location based on base sequences called restriction sites Bam HI ATGCGTGGCCGTACTACCCATGGATCCGGGTATATAG TACGCACCGGCATGATGGGTACCTAGGCCCATATATC ↓ 5’-GGATCC-3’ 3’-CCTAGG-5’ ↑ ↓ ↑

  4. Restriction Enzyme II: HaeIII ↓ 5’-GGCC-3’ 3’-CCGG-5’ ATGCGTGGCCGTACTACCCATGGATCCGGGTATATAG TACGCACCGGCATGATGGGTACCTAGGCCCATATATC ↑ ↓ ↑

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