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DNA Review. What does DNA store that is important? If a DNA strand read AGT-CCG-GTA what would the complimentary strand read? What holds the nitrogen bases together? What are the 3 components of a nucleotide?. DNA Replication. DNA Replication- the process of making a copy of DNA
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DNA Review • What does DNA store that is important? • If a DNA strand read AGT-CCG-GTA what would the complimentary strand read? • What holds the nitrogen bases together? • What are the 3 components of a nucleotide?
DNA Replication • DNA Replication- the process of making a copy of DNA • Why is this important? • Cells need to copy their DNA for mitosis (growth, repair, and maintenance) • Three steps: • Double helix unwinds and DNA helicase opens the strand • DNA polymerases add nucleotides back • Polymerases detach once replication is complete • You end with 2 stands of identical DNA strands
DNA Replication Step 1: • The double helix must unwind before replication can begin. • DNA helicases are enzymes that open the double helix by breaking the hydrogen bonds that hold the complimentary bases together (think of a zipper) • Replication goes from 5’ to 3’
DNA Replication Step 1 con’t: • Once separated, additional proteins hold the strands apart. • Where the double helix separates is called a replication fork (because of it’s Y shape)
DNA Replication Step 2: • At the replication fork enzymes called DNA polymerases move along each of the DNA strands. • DNA polymerases add nucleotides to the exposed bases according to the base pair rules
DNA Replication Step 3: • Step 2 continues until all DNA has been copied and the polymerases detach • Produces 2 DNA molecules that are identical to each other and the original strand