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Cultivation of viruses

E. &. O. O. &. Cultivation of viruses. Cultivation. To propagate the viral particle in its specific host i.e. Increase viral concentration through support viral replication. Cultivation. Bacteria. Animal. Insect. Plant. Methods of animal virus cultivation. Organized tissue.

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Cultivation of viruses

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  1. E & O O &

  2. Cultivation of viruses

  3. Cultivation To propagate the viral particle in its specific host i.e. Increase viral concentration through support viral replication.

  4. Cultivation Bacteria Animal Insect Plant

  5. Methods of animal virus cultivation • Organized tissue. • Cell culture. • Laboratory animals.

  6. Chick embryo technique

  7. Methods of animal virus cultivation • Organized tissue. • Cell culture. • Laboratory animals.

  8. Cell culture • Removal. • Homogenization. • Washing. • Trypsinization. • Washing. • Growing. • Inoculation. • Incubation.

  9. Methods of animal virus cultivation • Organized tissue. • Cell culture. • Laboratory animals.

  10. Intracerebrally Intraperitoneally Intramuscularly Intravenously Intranasally

  11. Types of cell culture Primary Permanent Suspension Diploid

  12. Primary cell culture A culture derived directly from a tissue

  13. Diploid cell culture Subsequent cultures derived from primary cell culture.

  14. Suspension cell culture A culture in it the dispersed cells are not allowed to settle down, but they are kept floating by continuous shaking of the flasks.

  15. Permanent cell line A culture derived from single separated cell i.e. diploid culture it can be propagated indefinitely.

  16. Cultivation of plant viruses • Protoplast and callus method. • Mechanical method. • Biological method.

  17. Cultivation of plant viruses • Protoplast and callus method. • Mechanical method. • Biological method.

  18. Inoculation Inoculation Incubation Incubation

  19. Cultivation of plant viruses • Protoplast and callus method. • Mechanical method. • Biological method.

  20. Cultivation of plant viruses • Protoplast and callus method. • Mechanical method. • Biological method.

  21. Incubation

  22. Cultivation of bacterial viruses 1.On broth media 2. On solid medium

  23. Virus + semisolid agar Bacteria + solid agar

  24. Cultivation of Insect viruses

  25. Thanks

  26. Criteria of purity Chemical purity Physical purity Biological purity

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