Download
1 / 12
120 likes | 242 Views
Proteins: Structure, Chemical Identification & Polypeptide Synthesis. Using Biuret to Identify Presence of a Protein Learning the Chemical Structure of an Amino Acid Using a Dehydration Synthesis to Make a Polypeptide Chaine. Testing for Organic Compounds Part A: Protein Testing.
E N D
Proteins: Structure, Chemical Identification & Polypeptide Synthesis Using Biuret to Identify Presence of a Protein Learning the Chemical Structure of an Amino Acid Using a Dehydration Synthesis to Make a Polypeptide Chaine
Testing for Organic CompoundsPart A: Protein Testing 1. Involves an indicator, Biruet Solution 2. Biuret reacts with the amino acids in the protein 3. Solution color goes from blue to violet 4. Color change indicates a chemical reaction between the biuret and the bonds between the amino acids of a protein
Another Look at Biuret Test Postive Control Experiment Negative Control Chemistry of the Biuret Test
Proteins • Are organic polymers composed of monomers called amino acids. • Strands of amino acids are called polypeptide chains. Where are the peptide bonds? Proteins are many chained structures.
Polypeptide Chain Amino Acids Protein with two Polypeptide chains Where is the complete protein?
Lab Safety & Cleanup • Test tubes are glass. • Biruet Solution is a base. Wash hands with water. • Goggles on at all times • Use special glassware soap and water with test tube brush to wash all test tube • Return all materials to appropriate location • Complete data table. • On back of lab, construct (5) observation statements and then write a conclusion.
Polypeptide Synthesis • Involves two amino acids. • Involves a dehydration synthesis. • Involves a chemical reaction that occurs between two specific areas of the amino acid. • Requires an –OH group and an –H from another –OH group
Functions of Proteins http://www.youtube.com/watch?v=iB7B0YaSLhU
