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Prevalence and resistance mutations of HIV-1 non-B subtypes among immigrants in Southern Spain

Abstract no. CDA051. Prevalence and resistance mutations of HIV-1 non-B subtypes among immigrants in Southern Spain. Beatriz de Felipe 1,3 , Pompeyo Viciana 1 , Luis F. López-Cortés 1 , Felipe Fernandez-Cuenca 2 , Mónica Trastoy 1 , Rosario Mata 1 , Manuel Leal 3 , Alejandro Vallejo 4,5.

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Prevalence and resistance mutations of HIV-1 non-B subtypes among immigrants in Southern Spain

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  1. Abstract no. CDA051 Prevalence and resistance mutations of HIV-1 non-B subtypes among immigrants in Southern Spain Beatriz de Felipe1,3, Pompeyo Viciana1, Luis F. López-Cortés1, Felipe Fernandez-Cuenca2, Mónica Trastoy1, Rosario Mata1, Manuel Leal3, Alejandro Vallejo4,5 Infectious Diseases Service, Hospital Virgen del Rocio, Seville, Spain. Microbiology Service Hospital Virgen Macarena Seville, Spain. Laboratory of Immunovirology, Infectious Diseases Service, Virgen del Rocío University Hospital, Seville, Spain. Laboratory of Molecular Virology, Infectious Diseases Service, Virgen del Rocío University Hospital, Seville, Spain. Laboratory of Molecular Virology, Infectious Diseases Service, Ramón y Cajal University Hospital, Madrid, Spain.

  2. Background • The HIV-1 non-B subtypes and recombinant forms are responsible for more than 95% of infections worldwide. • Most of the HIV-1 non-B subtypes are predominant in Sub-Saharan Africa and India. • The increase of immigration in Spain during the last few years has raised the prevalence of these subtypes with important consequences in treatment and transmission of the infection. OBJECTIVE The objective of this study was to analyse the prevalence of HIV-1 non-B subtypes among immigrants in the Southern Spain area since 2000. This study was approved by the Ethics Committee of the Hospital.

  3. PATIENTS: This cross-sectional study included 72 HIV-1-infected immigrants with available samples included in the dynamic open cohort of the Infectious Disease Service (SEI) of Virgen del Rocío Hospital, Seville, Spain. METHODS: The characterization of HIV-1 subtypes and drug resistance profile was performed by PCR and sequencing of the protease (PR) and retrotranscriptase (RT) genes. HIV subtyping and drug resistance mutations were determined using Stanford University Drug Resistance Database (http://hivdb.stanford.edu) and phylogenetic analysis comparing multiple published sequences using the UGENE software.

  4. Results I HIV-1 subtypes determined in our study: 1 CRF01_AE/ CRF02_AG 53 B 8 CRF02_AG 1 A 1 C 72 Patients were analyzed 2 CRF01_AE 1 J 1 F/B 1 F 2 CRF02_AG/G 1 G Most of the 72 immigrant patients showed HIV-1 subtype B (53/72; 76.6%) from South America and Western Europe. The remaining 19 patients had different HIV-1 non-B subtypes in PR and/or RT gene and were mostly from Sub-Saharan Africa and Eastern Europe.

  5. Results II This table shows the characteristics of the 19 immigrant patients infected with HIV-1 non-B subtypes. Four patients harbored different subtypes when PR and RT genes were compared. Only one patient had one protease inhibitor major resistance mutation (L90M), two patient had one RT major resistance mutation (K70R or K103N). ND: Not determinate HAART: Under HAART NO treatment: Previous HAART. Naïve: Not treatment antirretroviral never.

  6. Conclusion The prevalence of HIV-1 non-B subtypes has risen in Southern Spain since 2000. This could be an issue in the treatment and management of these patients. Most of these subtypes were from Sub-Saharan Africa and belong to the CRF02-AG subtype. Among them, only three patients presented major resistance mutations.

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