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Alu Polymorphysims as Identity Markers

Alu Polymorphysims as Identity Markers. Using PCR and Gel Electrophoresis. Alu Polymorphisms. Family of short interspersed elements (SINEs) 300 nucleotide length DNA segments Found primarily in the intron (non-coding) segments of DNA. Alu Polymorphisms. Found only in primates

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Alu Polymorphysims as Identity Markers

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  1. AluPolymorphysimsas Identity Markers Using PCR and Gel Electrophoresis

  2. Alu Polymorphisms • Family of short interspersed elements (SINEs) • 300 nucleotide length DNA segments • Found primarily in the intron (non-coding) segments of DNA

  3. Alu Polymorphisms • Found only in primates • Several are found only in humans • Specific human populations have unique frequencies of the Alu polymorphisms based on when the polymorphism was inserted by reverse transcriptase

  4. PV92 • Alu dimorphic SINE found on chromosome 16 • The two allele forms are ( + and - )

  5. PV92 • Population frequencies of PV92 is one method for reconstructing human evolution and migration patterns

  6. Polymerase Chain Reaction (PCR) • Technique used to amplify DNA • Use primers to select for segment of DNA • Simulates DNA replication using heat to denature double helix

  7. Gel Electrophoresis • Technique to visualize DNA segments • Uses electric current to move different sized pieces

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