1 / 3

PEG protocol

PEG protocol. as of April 2005. Cleaning, 2 hrs. Step A (Slides). Step B (Coverslips). Step C (Reaction containers). Step D, 1hr (Aminosilanization). Step E, 2hr incubation (PEGylation). Notes.

jaegar
Download Presentation

PEG protocol

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. PEG protocol as of April 2005

  2. Cleaning, 2 hrs Step A (Slides) Step B (Coverslips) Step C (Reaction containers) Step D, 1hr (Aminosilanization) Step E, 2hr incubation (PEGylation)

  3. Notes • Reaction containers: any glass containers which are used for aminosilanization. We recycle them. We use a couple of glass staining dishes and a flask. • Aminosilane: obtained from UCT, Cat. # A0700, 25mg, expire after six months • PEG: obtained from Nektar Therapeutics. biotin-PEG (0H4M0H02, BIO-NHS-5000), mPEG (2M4M0H01, M-SPA-5000) • Methanol: ACS grade • The other chemicals such as sodium bicarbonate: from Fisher • The diameter of our centrifuge is 5”. The power of our sonicator is 130W.

More Related