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Block Diagram

Block Diagram. Nikon (on-focal ) microscope. Electronic Box Including ： Voltage Follower Low-pass Filter Voltage Difference Amplifier. NI ： DAQ card (model # ： PCI-6010) & LabVIEW. PC LabVIEW software. PMT Signal. Operational Amplifier ： OP 27. Low noise, low drift, low offset

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Block Diagram

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  1. Block Diagram Nikon (on-focal ) microscope • Electronic Box • Including： • Voltage Follower • Low-pass Filter • Voltage Difference Amplifier NI： DAQ card (model #：PCI-6010) & LabVIEW PC LabVIEW software PMT Signal

  2. Operational Amplifier：OP 27 • Low noise, low drift, low offset • Supply voltage ( )：±12V (old design) / ±15V (new design)

  3. Voltage Follower Change the output impedance while the output voltage equals to the input one. Vsig Vout

  4. Butterworth Low-pass Filter • Synthesize a fourth-order Butterworth low-pass filter by cascading two second-order low-pass section. • Cut off Frequency: f0 80Hz • Ref：J. Millman, “microelectronics：digital and analog circuits & systems” pp.582-586 (1979) 10 k 1.52 k 10 k 12.35 k 2 k 2 k 2 k 2 k Vout Vsig 1μ 1μ 1μ 1μ

  5. Voltage Difference Amplifier • Amplifying the difference between two inputs by a gain factor. • Here Vref isadjustable offset voltage • Note that this circuit applies to negative signal input (old design) Rf R Vsig Vout +Vcc R Rf Vref -Vcc Vout = (Vref- Vsig)*

  6. Voltage Difference Amplifier • Amplifying the difference between two inputs by a gain factor. • Here Vref isadjustable offset voltage • Note that this circuits applies to positive signal input (new design) Rf +Vcc Vref R Vout -Vcc R Vsig Rf Vout = (Vsig - Vref)*

  7. 1.52K (502) Electronic Circuit Layout (old design) 12.35K (503) 10K 10K 1000K 100K 2K 2K 2K 2K +Vcc Signal Output ≦ 5 V 100K 1μ Signal Input (from detector) Signal ＜ 200 mV 1μ 1μ 1000K 1μ (503) -Vcc

  8. 1.52K (502) Electronic Circuit Layout (new design) 12.35K (503) 10K 10K 1000K 100K 2K 2K 2K 2K Signal Output ≦ 5 V 100K 1μ Signal Input (from detector) Signal ＜ 200 mV 1μ 1μ 1000K To panel potential meter 1μ

  9. R1 R2 Vsig Vsig Voltage Meter Driver • Non-inverting Amplifier • To drive the voltage meter ( Reason：to match the meter scale ) • Vout = Vin * ( ) = Variable Resistance (50K) Output

  10. Calibrate the Voltage Meter Scale • PMT, Monitor and the Voltage Meter should possess linear relationships • Adjust the gain (ratio of R1 / R2) of the non-inverting amplifier with different PMT values • PMT Voltage Range：0~1000V Monitor Signal Range：0~4V Voltage Meter Reading：0~10V • For example：

  11. Power Line Wiring in the Electronic Box (old design) L：red line N：blue line G：green line G 1 N L 2 switch 3 LNG -V +V com +5V LNG -V +V ±12V +15V

  12. Power Line Wiring in the Electronic Box (new design) L：red line N：blue line G：green line G 1 N L 2 switch 3 LNG -V +V com +5V ±15V

  13. Electronic and Panel Wiring (old design) To Electronic Circuit V+ V- +5V (not used) Vcom +12V -12V +15V Power Supply ±12V Power Supply Connect to the output of the voltage meter driver Connect to the input of the voltage meter driver To Electronic Circuit - + +15V Output (To PMT) Signal Output (To DAQ) PMT Signal Input PMT Voltage Monitor Signal Offset Voltage meter

  14. Electronic and Panel Wiring (new design) To Electronic Circuit +5V (not used) Vcom +15V -15V ±15V Power Supply Connect to the output of the voltage meter driver Connect to the input of the voltage meter driver To Electronic Circuit - + +15V Output (To PMT) Signal Output (To DAQ) PMT Signal Input PMT Voltage Monitor Signal Offset Voltage meter

  15. Electronic Testing • multi-meter： check the circuit before connecting to the power supply. • function generator & oscilloscope： check whether the output of each component satisfies our expectation： a. Each OP Amp should function properly！(make sure the pins have right voltage.) b. Voltage Follower：should be exactly the same with the input. c. Low-pass Filter：should filter out the signals with frequency higher than 80 hz. d. Voltage difference amplifier：should amplify our signals with the designed gain factor.

  16. IN Voltage Meter OUT 1 1 1 OFFSET 1 1 Circuit Layout 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 • 1 • 2 • 3 • 4 • 5 • 6 • 7 • 8 • 9 • 10 • 11 • 12 • 13 • 14 • 15 • 16 • 17 • 18 • 19 • 20 • 21 • 22 • 23 • 24 • 25 • 26 • 27 • 28 • 29 • 30 ：To V+ ：To V- ：To Vcom (ground) ：To the panel ：two points connected ：wired

  17. Testing the Frequency Response • With fixed amplitude sinusoidal wave signals, we can test how the electronic circuit box low-pass filter responds to the sinusoidal wave frequencies. Function Generator Oscilloscope Freq. OUTPUT Ch.1 Ch.2 Electronic Circuit Box Input Signal Output Signal

  18. Experimental Frequency Response • Input : 100 mV, sinusoidal waves -3 dB 75

  19. Part List in the Electronic Box

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