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Răspopescu Diana, seria B, gr.10 Tomită Georgiana, seria A, gr. 2

Răspopescu Diana, seria B, gr.10 Tomită Georgiana, seria A, gr. 2. Universitatea de Medicina si Farmacie ‘Victor Babes’ Timisoara . Efectul nanoparticulelor de aur asociate cu unde electromagnetice asupra celulelor maligne .

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Răspopescu Diana, seria B, gr.10 Tomită Georgiana, seria A, gr. 2

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  1. Răspopescu Diana, seria B, gr.10 Tomită Georgiana, seria A, gr. 2 Universitatea de Medicina si Farmacie ‘Victor Babes’ Timisoara

  2. Efectul nanoparticulelor de aur asociate cu unde electromagnetice asupra celulelor maligne

  3. Scopul studiului Gasirea unei metode pt distrugerea celulelor maligne Caracteristici : - neinvaziva - viabila pt distrugerea celulelor maligne - specificitate mare - toxicitate redusa

  4. Unde electromagnetice si nanoparticulele de aur • Celelele maligne pot fi distruse termic datorita undelor electromagnetice amplificate din mediu. • De ce nanoparticule de aur? • Ipoteza de nul a studiului: 1. GNPs-citotoxice 2. Celulele se dezvolta letal 3. Caldura produce injurii

  5. Metode • Laborator: GNPs 5nm-metode biochimice, filtrare (filtru de 0.22u). • In vitro 4 serii celulare maligne: Hep3B, Panc-1. • Serii control doar RF/serii RF+particule GNPs. • Studiu case contol/ test t pereche pt.: Diferite concentratii de GNPs-1, 10, 67 ul/l-Expunere la RF: 1, 2, 5 min, Analiza ME.

  6. Rezultate-citotoxicitatea • Viabilitate celule normale (fct. de C% GNPs): • Hep3B: • Panc-1: • 1uM/l => 100±5% • 10uM/l =>98±6% • 67uM/l => 86 ±8% • 1uM/l => 98±10% • 10uM/l =>90±11% • 67uM/l => 81 ±9%

  7. Rezultate-celule maligne distruse • Cel. maligne distruse -doar RF: -Pt GNPs(67uM/l)+RF-1,2,5 min~ 98% • Pt. 67um/l GNP+RF / RF => p=0.001 1 min- <20% 2min- <27%

  8. Valoarea P

  9. GNPs + RF

  10. Discutie • RFA conventional • Studii anterioare • GNPs-avantaje • De urmarit pe viitor

  11. Discutie • RFA conventional • Studii anterioare • GNPs-avantaje • De urmarit pe viitor

  12. Discutie • RFA conventional • Studii anterioare • GNPs-avantaje • De urmarit pe viitor

  13. Discutie • RFA conventional • Studii anterioare • GNPs-avantaje • De urmarit pe viitor

  14. Concluzii • GNPs nu prezinta efect citotoxic sau antiproliferativ pentru celulele umane. • GNPs asociate cu expunerea RF determina distrugerea termica celulelor maligne din tumorile gastrointestinale.

  15. Efectul GNPs+RF

  16. Referinte • 1. Bilchik AJ, Wood TF, Allegra D, TsioulRamming KP, Morton DL: Cryosurgical ablation and radiofrequency ablation for unresectable hepatic malignant neoplasms: a proposed algorithm. • 2. Bleicher RJ, Allegra, Bilchik AJ: Radiofrequency ablation in 447 complex unresectable liver tumors: lessons learned. • 3. Curley SA, Marra P, Beaty Early and late complications after radiofrequency ablation of malignant liver tumors in 608 patients. • 4. Haemmerich D, Laeseke PF: Thermal tumour ablation: devices, clinical applications and future directions. • 5. Gannon CJ, Curley SA: The role of focal liver ablation in the treatment of unresectable primary and secondary malignant liver tumors. • 6. Raut CP, Izzo F, Marra P, Ellis LM, Vauthey JN, Cremona F, Curley SA: Significant long-term survival after radiofrequency ablation of unresectable hepatocellular carcinoma in patients with cirrhosis.

  17. Va multumim!

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