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c. a. Heterogeneous Methylation. Single Nucleotide Changes. NNN T NNN. NNN C NNN. N CG N CG N CG N. N C G N CG N C G N. N CG N CG N C G N. NNN A NNN. NNN G NNN. N C G N C G N C G N. N G C N G C N G C N. N GC N GC N GC N. N G C N GC N G C N. N GC N GC N G C N. PCR. CREATES ssDNA.
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c a Heterogeneous Methylation Single Nucleotide Changes NNNTNNN NNNCNNN NCGNCGNCGN NCGNCGNCGN NCGNCGNCGN NNNANNN NNNGNNN NCGNCGNCGN NGCNGCNGCN NGCNGCNGCN NGCNGCNGCN NGCNGCNGCN PCR CREATES ssDNA BISULPHITE MODIFICATION NNNTNNN NNNCNNN NNNTNNN NNNCNNN * * NNNANNN NNNGNNN NNNGNNN NNNANNN NUGNUGNUGN NCGNUGNCGN NUGNUGNCGN NCGNCGNCGN NGUNGUNGUN NGCNGUNGCN NGUNGUNGCN MELT NGCNGCNGCN PCR - TARGET TOP STRAND -1ST DERIVATIVE -dF/dT NCGNCGNCGN NTGNTGNTGN NCGNTGNCGN NTGNTGNCGN FLUORESCENCE NGCNGCNGCN NACNACNACN NGCNACNGCN NACNACNGCN NCGNTGNCGN NTGNTGNCGN TEMPERATURE TEMPERATURE * * * * HETERODUPLEXES NACNACNGCN NGCNACNGCN b Homogeneous Methylation NCGNCGNCGN NCGNCGNCGN NGCNGCNGCN NGCNGCNGCN METHYLATION SENSITIVE HRM BISULPHITE MODIFICATION NUGNUGNUGN NCGNCGNCGN -1ST DERIVATIVE CREATES ssDNA NGUNGUNGUN NGCNGCNGCN -dF/dT FLUORESCENCE PCR - TARGET TOP STRAND NCGNCGNCGN NTGNTGNTGN TEMPERATURE TEMPERATURE NGCNGCNGCN NACNACNACN ordigitalMETHYLATION SENSITIVE HRM METHYLATION SENSITIVE HRM -1ST DERIVATIVE -1ST DERIVATIVE -dF/dT -dF/dT FLUORESCENCE FLUORESCENCE TEMPERATURE TEMPERATURE TEMPERATURE TEMPERATURE
