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Higher Resolution Mapping by Recombinant Frequency

Higher Resolution Mapping by Recombinant Frequency. need to produce a situation where every marker has an equal chance of transfer, so that recombination frequency is dependent only on distance between genesset up cross and select for entry of late marker

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Higher Resolution Mapping by Recombinant Frequency

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    1. Higher Resolution Mapping by Recombinant Frequency If markers are closely linked, they may not be resolved by time-of-entry mapping time-of-entry mapping also leads to biased recovery of early markers

    2. Higher Resolution Mapping by Recombinant Frequency need to produce a situation where every marker has an equal chance of transfer, so that recombination frequency is dependent only on distance between genes set up cross and select for entry of late marker� e.g. Hfr (leu+ arg+ met+) x F- (leu- arg- met-)

    4. Plasmids and Episomes The F factor is an example of a plasmid� a genetic element that is capable of self-replicating and existing independently of the bacterial chromosome It is also an example of an episome� a genetic factor capable of integrating into the bacterial chromosome

    18. Lateral Gene Transfer These methods of gene transfer are capable of transferring genetic information between related bacteria Genes are not required to be transferred by inheritance from one generation to the next There is evidence for lateral gene transfer through comparative genomic analysis of bacterial species thought to be distantly related

    21. Phage Classifications Virulent phage: kill the host�will always undergo a lytic infection cycle. Temperate phage: can integrate into the host cell chromosome (form a prophage)�capable of lysogenic infection cycle.

    27. Biochemical Nature of the Gene Experiments with mutant strains of Streptococcus pneumoniae (pneuococcus), F. Griffith, 1928. rough: nonpathogenic strain; bacteria unable to synthesize polysaccharide coat smooth: pathogenic strain: polysaccharide coat allows cells to escape immune surveillance

    77. If DNA polymerase can�t snythesize a strand de novo, what starts DNA synthesis? A complex of proteins assembles at the the replicating fork; one of those enzymes is an RNA polymerase that synthesizes an RNA primer...

    101. Retrotransposons (Hartwell pp. 460-64) Some elements that can move within eukaryotic genomes (transposition) do so using an RNA intermediate e.g. TY elements in yeast; copia elements in Drosophila like viruses, these movable elements can result in mutations a large number of spontaneous mutations in Drosophila arise from this mechanism...

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