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Chapter 26 Microbial Growth Control

Chapter 26 Microbial Growth Control. Physical Antimicrobial Control. Clicker Question: Clicker Question:. Physical Antimicrobial Control. Clicker Question: Clicker Question:. Thermal Death Curve: Mesophilic Bacterium. Clicker Question:. Thermal Death Curve: Mesophilic Bacterium.

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Chapter 26 Microbial Growth Control

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  1. Chapter 26Microbial Growth Control

  2. Physical Antimicrobial Control Clicker Question: Clicker Question:

  3. Physical Antimicrobial Control Clicker Question: Clicker Question:

  4. Thermal Death Curve: Mesophilic Bacterium Clicker Question:

  5. Thermal Death Curve: Mesophilic Bacterium Clicker Question:

  6. Thermal Death Curve: Mesophilic Bacterium Clicker Question:

  7. Thermal Death Curve: Mesophilic Bacterium Clicker Question:

  8. Decimal Reduction Time vs Temperature Clicker Question:

  9. Pasteurization Times and Temperatures: Pasteur’s original: half hour at 63oC-66oC as a Batch. Originally designed to prevent spoilage of wine. Later applied to milk to prevent Mycobacterium bovis transmission of TB. Milk current standard based on killing Listeria monocytogenes, Campylobactersps, Salmonella and Escherichia coli O157:H7, Coxiellaburnetti. HEAT EXCHANGER: 72oC for 15 sec. Clicker Question:

  10. HTST Pasteurization – Continuous Counter Current Flow Clicker Question: Counter-current Heat Exchanger

  11. Small Heat Exchanger Small Heat Exchanger

  12. Heat Exchanger Diagram

  13. Ultrapasteurization Heat Exchanger : heats milk to 138oC for 3 sec (2 to 4 sec). Ultrapasteruized milk is supposed to last 6 months at room temperature without spoilage. Milk is packaged in plasticized cardboard containers. There’s a Story Here.

  14. The Autoclave and water !

  15. Ultra Violet Lights Are ON The mesh work at the back holds behind it a HEPA Filter so that sterile air moves outward as a laminar flow. Clicker Question:

  16. Radiation Kills Just Like Heat 1 Gray (Gy) = 100 rads 1 rad = 100 ergs/g

  17. Decimal Reduction Doses of Ionizing Radiation Check out the D10 for humans

  18. Clicker Question:

  19. It Works Beautifully with Food

  20. Filtration Depth Filters Membrane Filters Nucleopore Filters.

  21. Bacteria stuck on membrane filters. Clicker Question:

  22. Membrane Filtration Clicker Question:

  23. Antimicrobial Chemical Effects

  24. Minimal Inhibitory Concentration Lowest Concentrations    Highest Concentrations We did these with microtiter plates in Chapter 31 (Fig 31.8)

  25. Antiseptics

  26. Antimicrobial Agents Used In Vivo Clicker Question:

  27. Antimicrobial Spectra Clicker Question:

  28. Antibiotic Production Today

  29. Salvarsan and Paul Ehrlich Clicker Question:

  30. Sulfa Drugs: Gerhard Domagk, 1930’sFirst Chemosynthetic Antibiotics

  31. Nucleoside Analogs First ones were simple, more complex one developed in response the AID’s epidemic.

  32. Quinolones Ciprofloxacin Moxifloxacin Clicker Question:

  33. Naturally Occurring Antimicrobials:The Antibiotics

  34. β-lactamase Resistant CeftriaxoneA Cephalosporin

  35. The Amino Glycosides: Target 30S ribosome

  36. The Macrolides : EythromycinTargets the 50S ribosome

  37. The Tetracyclines: Targets 30 S ribosome

  38. Daptomycin and Platensimycin Inhibits Fatty Acid biosynthesis Cyclic Lipopeptide: alters Cell Membranes

  39. Antibiotic Resistance Antibiotic Resistance Mechanisms Lacks structure antibiotic inhibits. Impermeability. Enzyme that alters structure of antibiotic. Makes modification of antibiotic target. Develops a resistant, alternate pathway. Efflux Pump.

  40. The Classics !

  41. Epidemiological Patterns of Antibiotic Resistance

  42. Appearance of Drug Resistance

  43. Resistance to Vancomycinover come by converting the carbonyl to a methyl.

  44. Computer Aided Drug Design

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