PathogenCombat Objective

1. Control and Prevention of emerging and future pathogens at cellular and molecular level throughout the food chain Prof. Mogens Jakobsen Dept. of Food Science The Royal Veterinary and Agricultural University, Copenhagen, Denmark (mogens.jakobsen@kvl.dk) Coordinator of PathogenCombat

2. PathogenCombat Objective To provide new essential information and methods to the food industry and public authorities on how to reduce the prevalence of new and re-emerging foodborne pathogens

3. Pathogens

4. HIGH TECHNOLOGY PLATFORMS Bioimaging, Flourescent Ratio Imaging Microscopy (FRIM), Force Scope, Confocal Laser Scanning Microscopy (CLSM), Atomic Force Microscopy (AFM) Pathogen matrix interactions

5. HIGH TECHNOLOGY PLATFORMS Mamalian functional cell models to replace experimental animals Host-pathogen interaction

6. HIGH TECHNOLOGY PLATFORMS Functional genomic and bioinformatic based development of DNA-microarrays for pathogen detection viability/stress response/virulence Detection and monitoring

7. HIGH TECHNOLOGY PLATFORMS High Throughput Screening (HTS) for thousands of protective and probiotic cultures Robotic system

8. HIGH TECHNOLOGY PLATFORMS Identification of CCP by use of stochastic simulation models based upon quantification of numbers of pathogens including mycotoxin producing fungi and their virulence/toxcicity expression along the entire food chain Estimation of microbial food safety risk at time of consumption based upon stochastic simulation modelling of number of pathogens and their virulence/toxcicity expression Modelling microbial population behaviour throughout the food chain

9. PathogenCombat Deliverable I + II New approach to estimate the prevalence of virulent pathogens up to the time of consumption New methods to control the viability and virulence of pathogens throughout the food chain until the time of consumption

10. FRIM

11. 12 Min 0 Min FRIM - Simultaneous detection of physiological status by intracellular pH-determination Listeria monocytogenes

12. Force scope – quantifying the microbial adhesion by a novel technique

13. New method for estimation of host-pathogen interaction based upon functional cell models PathogenCombat Deliverable III

14. Novel processing technologies to inactivate pathogens New probiotic and protective cultures to eliminate pathogens Elimination of biofilms based upon hygienic design PathogenCombat Deliverable IV

15. PathogenCombat Deliverable V - VII Established strategies and actions for disseminating project deliverables for food SMEs New mathematical models covering food at time of consumption New cost effective food safety management systems

16. Improved awareness of food safety for the European consumer, food industries and regulatory agencies PathogenCombat Deliverable VIII

18. NTC I NTC II NTC III RTD I RTD III RTD IV RTD II Discovery and Development Detection & Virulence traits Control & Prevention Application and Management SME network Training activities Consumer awareness, food industry and regulatory agencies PathogenCombat 5 year Integrated Project Total budget 14.3 Mio € EU grant to the budget 11,3 Mio €

19. Training • Training of SME food producing partners and implementation of project deliverables • Workshop for about 150 European, INCO and Accession Country SMEs and regulatory agencies • Pan European dissemination of deliverables via generic food safety management system • Exchange of scientists (40 + 10 INCO/Accession Countries)