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PulseNet Latin America (PN-LA)

PulseNet Latin America (PN-LA). 5th International Meeting. 23 to 25 de october, 2007. PulseNet Latin America: Updating. Enrique Perez Norma Binsztein. PulseNet Latin America (LA). Objective:.

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PulseNet Latin America (PN-LA)

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  1. PulseNet Latin America (PN-LA) 5th International Meeting 23 to 25 de october, 2007 PulseNet Latin America: Updating Enrique Perez Norma Binsztein

  2. PulseNet Latin America (LA) Objective: To reduce the Diseases of Food Transmission Promoting the utilization of Molecular Techniques for the diagnosis and typing of pathogens in order to strengthen surveillance and the system of response in the countries of Latin America, being part of the PulseNet International Network

  3. Molecular Epidemiology Powerful tool for Laboratory Based Surveillance FBD = Integrated Surveillance

  4. PulseNet LA Specific objectives Strengthen National and Regional Surveillance of FBDs Improve the recognition, diagnosis, and investigation of outbreaks Detect early emerging and re-emerging pathogens Establish National and Regional Databases Strengthen communication between countries Use carefully the National and Regional Information in actions and health coordinated interventions Consolidate the collaboration between Laboratory, Epidemiology and Clinical Benefits for establishing the PulseNet Network in LA

  5. PulseNet LA History of the Network (I) 2003 - Agreement to develop PN in Latin America -1st training Course and 2nd Meeting. Seven countries: Brazil, Chile, Colombia, Mexico, Argentina, Uruguay and Venezuela. - Operation regulation Terms of Reference for the Network - Process of certification: `Salmonella` and E. coli O157. Beginning - 2nd Course training and 3rd Meeting. Six countries are incorporated: Bolivia, Cuba, Costa Rica, Nicaragua, Paraguay and Peru 2004 2005

  6. PulseNet LAHistory of the Network (II) 2006 - 3rd Training Course and 4th Annual Meeting Process of certification and proficiency Continues Salmonella and E. coli O157 Other (Campylobacter, Shigella sonnei, Lysteria monocytogenes, Vibrio cholerae) 2006-2007

  7. Who participate in thePulseNet LA • Interest of the country and of the National Reference Laboratory Incorporation for Surveillance, Control and FBD Prevention • Active involvement in the Pathogen Monitoring of Foodborne transmission • Participation in the EQAS program (Quality Control) of the WHO Global Salmonella Surveillance • Submission of application to the Steering Committee of PulseNet LA

  8. Data from surveillance, Available in countries National database outbreaks, projects Client Admin PFGE Equipment and Software Regional Center Databases Management Regional database Not-available in countries Surveillance, outbreaks, projects Client Results • Regional Center • PFGE • Analysis National Database (Regional Center)

  9. PulseNet Latin AmericaParticipants Mexico Cuba Nicaragua Costa Rica Venezuela Colombia Brazil (2) Perú Bolivia Paraguay Chile Uruguay Argentina

  10. PulseNet LASituation up to October2007 Nº of participants 14 National Reference Laboratories of 13 countries 11/14 Cheff (10) Another (1) Guefast 06 6/14 BioNumerics (4) GelCompar (2) Equipment Software

  11. PulseNet LACertification 1. Salmonella and E. coli O157 2. Other microorganisms–Regional Center Gels and Analysis - Campylobacter - Listeria monocytogenes - Shigella sonnei - Vibrio cholerae

  12. PulseNet LAActivities 2006 - 2007 • Studies conducted by the participants of the Network • Response to international requests of information • Regional Database • Collaborations among the participants (intra Network) • Participation in the meeting of the PulseNet International Steering Committee. Providence, USA, 16 April 2007 • Participation in the Shigella flexneri working group for the PFGE protocol • Development of a new protocol for Enterobacter sakazakii PFGE

  13. PulseNet LASurveillance and outbreaks of FBD. Studies conducted by 12/14 participants of the Network Monitoring Surveillance Systems Network of Laboratories = 11/12 Sentinel Units = 5/12 Mandatory notification = 7/12 E. coli and/or Salmonella and/or Shigella Study of outbreaks, 2004-2006 # outbreaks # outbreaks-PFGE observations (participants) (participants) Salmonella 134 (8/12) 64 (6/12) *S Typhi, Typhimurium, Enteritidis, other Shigella 39 (3/12) 11 (1/12) Sh flexneri, Sh sonnei, Sh boydii E. coli O157 5 (1/12) 5 (1/12) Lab Surveillance and PFGE, 2006-2007 participants # isolations observations Salmonella 8/12 ** 745 STyphi, Typhimurium, Enteritidis, other E. coli 3/12 619 O157(332) and no-O157 (287) Others PTA 2/12 213 Sh sonnei, Campylobacter and other *, **: PFGE of 1 and 2 participants, respectively, carried out in the INEI

  14. PulseNet LA Activities 2006-2007International requests of information and responses of PN-LA 1. PulseNet International 1.a.S Muenchen and S Wandsworth - isolates in WGSS South and Central America response of 17 countries 1.b.outbreak of S Enteritidis in Qatar response of 3 participants PN-LA 1.c.S Typhymurium in Pennsylvania, USA response of 2 participants PN-LA 1.d.Salmonella I4,[5] .12:i:-, outbreak in USA, October 2007 2. INFOSAN Shigella sonnei outbreak in Denmark. Answer of the Regional Center Results of the experience: How to continue

  15. PulseNet LA Activities 2006-2007Regional DataBases (RDB) • Training at CDC, December 2006 (Mariana Picheland Isabel Chinen) Objectives: operation, interrelationship between National Databases and Regional Database. Organization and transmission of the information b) Interconnection with the RDB: data entry, information comparison and acquisition. Technical difficulties in the connection and configuration with the Server Central PAHO/WHO to strengthen 2007-2008 • Income of 110 patterns of S. Enteritidis Transfer to the LA Network (5th Meeting)

  16. PulseNet LA Activities 2006-2007Collaboration intra Net 1. Visits of technical cooperation Regional Center 2. Creation of National Data Bases of participants Labs Two in the Regional Center (Salmonella) 3.Continuous technical assistance 2005: Uruguay and Venezuela 2006: Mexico and Perú

  17. PulseNet LA Technical group of theRegional Center–Octubre2007 - Supervision Salmonella, Shigella, Campylobacter, Vibrio cholerae: Mariana Pichel Escherichia coli: Isabel Chinen - Database Administration Escherichia coli: Isabel Chinen; Carolina Carbonari Salmonella: Angela Salve; Mariana Pichel Shigella sonnei, Campylobacter: María Rosa Viñas; Mariana Pichel Vibrio cholerae: María Rosa Viñas Lysteria monocytogenes: Claudia Martinez - Administrative collaboration Lorena Chaparro

  18. PulseNet LA Where are we and how to followProposals for the 5th discussion Meeting • Intensify Surveillance of PTA and study of outbreaks of FBD by PFGE “per se” or in collaboration 2. Creation and consolidation of National Databases “per se” or in collaboration 3. Operation of Regional Database 4. Communication among the members of the Network. Information. Definition of tools and timetable of “encounters” 5. Pending certifications: when and who (in accordance with the operating capacities) 3 participants = gels Salmonella and/or E coli 3 participants = gels partial Salmonella 1 participant = gels and analysis Salmonella 3 participants = analysis Salmonella and/or E.coli O157

  19. PulseNet LA Where are we and how to followProposals for the 5th discussion Meeting (cont) 6. Beginning process of Periodic Proficiency test Who/ When/ Microorganism 7. Incorporation of new pathogens Campylobacter/ Vibrio cholerae/ Vibrio parahaemolyticus/ Lysteria monocytogenes/ Shigella sonnei Which? Who? Timetable of certifications 8. Incorporation of new participants 9. Commitments

  20. PulseNet LA Acknowledgements Participants of the 14 National Reference Laboratories Technical Group of the Regional Center Members of PN-International and its coordinator (Ahmed Elsedawy) Coordinators of PN-USA, CDC (Peter Gerner Smidt), laboratory (Efrain Ribot, Kara Cooper and Mary Ann Fair) and of administration of databases (Kelley Hise) Technical group of Informatics in Regional Bases (Alberto Mendia) in the CDC (Brenda Brown) and in the INEI (Ciro Rasmussen) PAHO/WHO in Argentina Dr. Antonio Pagés and Dr. Celso Rodriguez FOS-OPS/OMS

  21. Thank you very much

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