Rotating Vessel Wall For the Adhesion of Cardiac Cells

1. Rotating Vessel Wall For the Adhesion of Cardiac Cells [Roli Kumar] [Advisor: Dr. Marc Lin]

2. Overview of Last Presentation • Importance of building a rotating vessel for replacement of damaged heart tissue and drug therapy research • objective was to develop 3-D cardiac tissue from a monolayer of cardiac cells • Basic design specifications of the rotating vessel

3. Apparatus Set-Up Suction cups Plastic Rod Plastic/metal Rotating vessel 2.5cm 1.5cm 1.25cm Magnetic Stirrer

4. How many cells are needed? • 1.6X 10^5 cells/cm^2 • surface area= SA of cylinder+SA half circle • SA=2(pi)rh +.5(4(pi)r^2) • No. of cells= SA sphere X (cell/cm^2) • No. of cells= 3,455,200 cardiac cells

5. Cardiomyocytes • Chick embryos • Chicks will be 7-8 days old • higher disassociation rate with younger chicks • older chicks produce low yield in disassociated cells

6. Rotating Vessel • Metal ball • collagen will not bind • Plastic ball • collagen will bind • rat collagen • will not rotate with magnetic stirrer • aluminum foil

7. Design Specifications • End-over end mixing • measurements at various speeds • problem: too fast, too slow • Solid body rotation • suspending cells in a liquid medium • inject the cells in • minimizing air bubbles

8. Design Specifications • Turbulence • suction cups • tubes connected • Aeration • medium usually contain bicarbonate • produces CO2 • HEPES • not a bicarbonate buffer

9. Work Completed • Learned procedure for Cardiac Myocyte Isolation Protocol • cells were infected with bacteria

10. Current Work • Repeat procedure using the ventricular portion of the heart • add penicillin to prevent bacterial growth • Sanitize rotating vessel • Put apparatus together

11. Future Plan of Action • Measure the speed of the rotating vessel • Model the forces applied on the cell