1 / 11

Analysis of a Double-Strand DNA Break in living S. cerevisiae Cells

Analysis of a Double-Strand DNA Break in living S. cerevisiae Cells. Leana M. Topper Kerry S. Bloom. Department of Biology, University of North Carolina at Chapel Hill;. Chromosome III. MAT. CEN. ~85 kb. LacO array. ~500 bp. MAT. LacO. KpnI. KpnI. probe. 2.5 kb.

kayla
Download Presentation

Analysis of a Double-Strand DNA Break in living S. cerevisiae Cells

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Analysis of a Double-Strand DNA Break in living S. cerevisiae Cells Leana M. Topper Kerry S. Bloom Department of Biology, University of North Carolina at Chapel Hill;

  2. Chromosome III MAT CEN ~85 kb LacO array ~500 bp

  3. MAT LacO KpnI KpnI probe 2.5 kb No pGalHOT W/ pGalHOT 0 0.5 2 3 4 1 0 0.5 2 3 4 1 h post gal 8 kb KpnI fragment HO cut fragment

  4. 6:00 7:00 2:00 10:00 13:00 15:00 8:00 Live cell after HO induction

  5. Movement of Spindle Pole Bodies and lacO in live cells following HO expression Average film time: 13 min No bud: 13 Small-budded cells: 8 Large-budded cells: 29 Average spindle length = 1.69 ±0.32mm Range = 1.05-2.44 mm

  6. Population analysis of lacO and SPBs after induction of HO

  7. Deletion of Rad52 does not affect LacO movement 6:00 2:00 0:00 9:00 21:00 15:00 20:00

  8. Formation of Rad52 foci following DNA damage

  9. Formation of Rad52-GFP foci after induction of HO

  10. Rad52-GFP foci and Spindle Pole Bodies Move Independently 7:00 5:00 1:00 17:00 18:00 8:00 12:00

  11. Rad52-CFP and LacO spots do not colocalize 6:00 8:00 3:00 16:00 9:00 11:00 14:00

More Related