Silicon-based minerals for ecological agriculture

1. Silicon-based minerals for ecological agriculture Natalia Zaimenko, Ecormin-Planet, Ukraine 2008

2. Silicon – Background Information • Participates actively in pollination processes • Increases photosynthesis productivity • Protects plants from pests and deceases • Improves plants adaptability to stress-factors • Increases crops productivity • Diminishes negative impact of soil salinity on plants • Improves water supply to plants Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

3. The Problem Research of effects of analcinite (silicon-containing mineral) on: • ecological, physiological and biochemical processes in plants • Dynamics of macro- and micro-elements distribution in soil • Microbiological activity and toxicity of soil Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

4. Chemical Composition of Analcinite,the masses. % , Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

5. TPD MS thermograms of H2O (1)and CO2 (2)evolution for samples of various dispersiveness a Fig.1. TPD MS thermograms for two components of mass spectra: H2O (1); CO2 (2) for two samples of analcinite, industrial dispersion; average particle size 0.5 mm. Fig.2. TPD MS thermograms for two components of mass spectra: H2O (1); CO2 (2) for the sample of analcinite, special dispersion, average particle size 3 mcm. Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

6. TPD MS thermograms of H2O (1) и CO2 (2)evolution for analcinitesamples after rehydratation and for trepel of average particle size 20 mcm Fig.3. Two components of mass spectra: H2O (1); CO2 (2) after re-hydration of the analcinite. Re-hydration included heating the sample up to 8000C, cooling and further saturation by water vapour. Fig. 4. TPD MS thermograms for the two components of mass spectra: H2O (1); CO2 (2) for of trepel. Special dispersion down to the average particle size about 20 mcm. Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

7. Activity of Redox Enzymes in Winter Wheat on Analcinite Background Activity of polyphenol oxidase А – change of optic density of pirokatechine 60 sec per 1 g of raw matter Activity of cytochrome oxidase А – change of optic density of phosphate buffer 30 sec per 1 g of raw matter Activity of peroxidase А – change of optic density of purplegaline 1 sec per 1 g of raw matter Activity of ascorbic oxidase А – change of optic density of phosphate buffer 60 sec per 1 g of raw matter Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

8. 2943 3000 2500 2238 2000 1654 1476 1422 1354 1391 RNA 1500 DNA 1000 500 156 87 75 61 60 46 45 0 Control Powdering of seeds 50 100 200 300 400 Analcinite doze Content of Nucleic Acids in Maize Growthes Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

9. 35 30.3 30.0 29.1 30 25 22.4 19.6 19.0 18.9 20 Numerical index 15 10 5 0 Control Powdering of seeds 50 100 200 300 400 Analcinite doze Numeric Index of Structural State of Maize Genome Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

10. Analcinite Effect on Activity of Redox Enzymes in Leaves of Winter Wheat and Sugar-Beet Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

11. Variation of Amino Acid Content in Leaves of tested Plants after Analcinite Application, mkg/100 mg of raw matter Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

12. Analcinite Effect on Content of Macro- and Microelements in Soil, 1 N HCl Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

13. Variation of Elements-Biophils in Winter Wheat and Sugar-Beet Plants (macroelements, %; microelements, mg/kg of ash) Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

14. Analcinite Effect on Enzymatic Activity of Soil under Winder Wheat and Sugar-Beet Cultivation Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

15. Analcinite Effect on Soil Toxicity and Content of Phytotoxic Microflora after Winter Wheat and Sugar-Beet Cultivation Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

16. Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

17. Efficiency of Organic-Minerals for Sugar-beet Cultivation (2007) Test. Development of sugar beets from seeds panned and incrustated by analcinite Control. Development of sugar beet from seeds panned and incrustated by typical mixture Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

18. Summary Analcinite • Promotes activity of physiological-biochemical processes in plants at the earliest stages of their development • Promotes increase of plants adaptability to stress-factors • Improves supply of biogenic elements to plants • Improves enzymatic soil activity, microbiota development • Decreased toxicity of soil and phytotoxicmicroflora development • Decreases nucleides accumulation in crops The results are proven by laboratory and vegatative experiments, as well as field experiments of 2006-2007 on the area of 250ha Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

19. Future Plans • Research of analcinite influence on vine resistance to fungi and bacteria • Analcinite introduction into nanotechnology in plant-growing • Research of analcinite influence on “nucleotic base-biotic radical of amino acid” liason Natalia Zaimenko - Ecormin Planet - Ukraine - 2008

20. Contacts:Zaimenko Natalia Doctor of Biological Science,Director of National Botanic Garden of Ukraine, named afterGryshko1, Tymiriazivs’ka Str. Kyiv, 01014 UkraineTel. +38 (044) 285 41 05MaistrenkoSvitlana“Ecormin-Planet Ltd.”Tel/fax: +38 044 285 02 14 E-mail: office@ecormin.com Natalia Zaimenko - Ecormin Planet - Ukraine - 2008