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Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies

GEIL-GTLLF 2008. Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies. Part one : Leukocyte subsets A colour code is applied trhoughout this atlas: Granulocytes in red Monocytes in green Lymphocytes in purple

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Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies

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  1. GEIL-GTLLF 2008 Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies Part one : Leukocyte subsets A colour code is applied trhoughout this atlas: Granulocytes in red Monocytes in green Lymphocytes in purple All other cells, in a region of maturation defined by the exclusion of mature cell types and thus dubbed « bermudes » in cyan I.1

  2. Combinations Tube 1 : CD11b/CD14/CD16/CD45 Tube 7 : HLADR/CD117/CD34/CD45CD11b/CD14 p. I-5 HLADR/CD117 p. I.29 CD11b/CD16 p. I-6 DR/CD34 p. I.30 CD14/CD16 p. I-7 CD117/CD34 p. I.31 Tube 2 :CD11b/CD117/CD34/CD45 Tube 8 : CD34/CD56/CD19/CD45 CD11b/CD117 p. I-9 CD34/CD56 p. I.33 CD11b/CD34 p. I-10 CD34/CD19 p. I.34 CD117/CD34 p. I-11 CD56/CD19 p. I.35 Tube 3 :CD36/CD71/CD11c/CD45 Tube 9 : CD64/CD24/CD10/CD45 CD36/CD71 p. I-13 CD64/CD24 p. I.37 CD36/CD11c p. I-14 CD64/CD10 p. I.38 CD71/CD11c p. I-15 CD24/CD10 p. I.39 Tube 4 : CD34/CD13/CD33/CD45 Tube 10 : CD7/CD117/CD34/CD45 CD34/CD13 p. I-17 CD7/CD117 p. I.41 CD34/CD33 p. I.18 CD7/CD34 p. I.42 CD13/CD33 p. I.19 CD117/CD34 p. I.43 Tube 5 : CD38/HLADR/CD34/CD45 Tube 11 : CD22/CD10/CD20/CD45 CD38/HLADR p. I.21 CD22/CD10 p. I.45 CD38/CD34 p. I.22 CD22/CD20 p. I.46 HLADR/CD34 p. I.23 CD10/CD20 p. I.47 Tube 6 : CD38/CD10/CD19/CD45 Tube 12 : CD4/CD2/CD56/CD45 CD38/CD10 p. I.25 CD4/CD2 p. I.49 CD38/CD19 p. I.26 CD4/CD56 p. I.50 CD10/CD19 p. I.27 CD2/CD56 p. I.51 I.2

  3. SSC FLy SSC FLy FLy FLx FLx CD45 FLx FSC Leukocyte subsets display FLy FLx I.3

  4. CD11b/CD14/CD16/CD45 TUBE 1 I.4

  5. SSC SSC FLy CD11b/CD14 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD14PE FSC-Height CD45APC CD11bFITC CD14PE CD14PE CD14PE CD11bFITC CD11bFITC CD11bFITC I.5

  6. SSC SSC FLy CD11b/CD16 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD16PC7 FSC-Height CD45APC CD11bFITC CD16PC7 CD16PC7 CD16PC7 CD11bFITC CD11bFITC CD11bFITC I.6

  7. SSC SSC FLy CD14/CD16 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD16PC7 FSC-Height CD45APC CD14PE CD16PC7 CD16PC7 CD16PC7 CD14PE CD14PE CD14PE I.7

  8. CD11b/CD117/CD34/CD45 TUBE 2 I.8

  9. SSC SSC FLy CD11b/CD117 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height FSC-Height CD45APC CD45APC CD117PE CD117PE CD117PE CD11bFITC CD11bFITC CD11bFITC I.9

  10. SSC SSC FLy CD11b/CD34 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD34PC5 FSC-Height CD45APC CD11bFITC CD34PC5 CD34PC5 CD34PC5 CD11bFITC CD11bFITC CD11bFITC I.10

  11. SSC SSC FLy CD117/CD34 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD34PC5 FSC-Height CD45APC CD117PE CD34PC5 CD34PC5 CD34PC5 CD117PE CD117PE CD117PE I.11

  12. CD36/CD71/CD11c/CD45 TUBE 3 I.12

  13. SSC SSC FLy CD36/CD71 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD71PE FSC-Height CD45APC CD36FITC CD71PE CD71PE CD71PE CD36FITC CD36FITC CD36FITC I.13

  14. SSC SSC FLy CD36/CD11c FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD11cPC5 FSC-Height CD45APC CD36FITC CD11cPC5 CD11cPC5 CD11cPC5 CD36FITC CD36FITC CD36FITC I.14

  15. SSC SSC FLy CD71/CD11c FSC FLx CD45 FLy FLy FLy FLx FLx FLx CD11cPC5 SSC-Height SSC-Height FSC-Height CD45APC CD71PE CD11cPC5 CD11cPC5 CD11cPC5 CD71PE CD71PE CD71PE I.15

  16. CD34/CD13/CD33/CD45 TUBE 4 I.16

  17. SSC SSC FLy CD34/CD13 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD13PE FSC-Height CD45PC7 CD34FITC CD13PE CD13PE CD13PE CD34FITC CD34FITC CD34FITC I.17

  18. SSC SSC FLy CD34/CD33 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD33APC FSC-Height CD45PC7 CD34FITC CD33APC CD33APC CD33APC CD34FITC CD34FITC CD34FITC I.18

  19. SSC SSC FLy CD13/CD33 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD33APC FSC-Height CD45PC7 CD13PE CD33APC CD33APC CD33APC CD13PE CD13PE CD13PE I.19

  20. CD38/HLADR/CD34/CD45 TUBE 5 I.20

  21. SSC SSC FLy CD38/HLADR FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height HLADRPE FSC-Height CD45ECD CD38FITC HLADRPE HLADRPE HLADRPE CD38FITC CD38FITC CD38FITC I.21

  22. SSC SSC FLy CD38/CD34 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD34PC5 FSC-Height CD45ECD CD38FITC CD34PC5 CD34PC5 CD34PC5 CD38FITC CD38FITC CD38FITC I.22

  23. SSC SSC FLy HLADR/CD34 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD34PC5 FSC-Height CD45ECD HLADRPE CD34PC5 CD34PC5 CD34PC5 HLADRPE HLADRPE HLADRPE I.23

  24. CD38/CD10/CD19/CD45 TUBE 6 I.24

  25. SSC SSC FLy CD38/CD10 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD10PE FSC-Height CD45Cy7 CD38FITC CD10PE CD10PE CD10PE CD38FITC CD38FITC CD38FITC I.25

  26. SSC SSC FLy CD38/CD19 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD19Cy5 FSC-Height CD45Cy7 CD38FITC CD19Cy5 CD19Cy5 CD19Cy5 CD38FITC CD38FITC CD38FITC I.26

  27. SSC SSC FLy CD19/CD10 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD19Cy5 FSC-Height CD45Cy7 CD10PE CD19Cy5 CD19Cy5 CD19Cy5 CD10PE CD10PE CD10PE I.27

  28. HLADR/CD117/CD34/CD45 TUBE 7 I.28

  29. SSC SSC FLy HLADR/CD117 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD117PE FSC-Height CD45Cy7 HLADRFITC CD117PE CD117PE CD117PE HLADRFITC HLADRFITC HLADRFITC I.29

  30. SSC SSC FLy HLADR/CD34 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD34Cy5 FSC-Height CD45Cy7 HLADRFITC CD34Cy5 CD34Cy5 CD34Cy5 HLADRFITC HLADRFITC HLADRFITC I.30

  31. SSC SSC FLy CD117/CD34 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD34Cy5 FSC-Height CD45Cy7 CD117PE CD34Cy5 CD34Cy5 CD34Cy5 CD117PE CD117PE CD117PE I.31

  32. CD34/CD56/CD19/CD45 TUBE 8 I.32

  33. SSC SSC FLy CD34/CD56 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD56PE FSC-Height CD45APC CD34FITC CD56PE CD56PE CD56PE CD34FITC CD34FITC CD34FITC I.33

  34. SSC SSC FLy CD34/CD19 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD19PC7 FSC-Height CD45APC CD34FITC CD19PC7 CD19PC7 CD19PC7 CD34FITC CD34FITC CD34FITC I.34

  35. SSC SSC FLy CD56/CD19 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD19PC7 FSC-Height CD45APC CD56PE CD19PC7 CD19PC7 CD19PC7 CD56PE CD56PE CD56PE I.35

  36. CD64/CD24/CD10/CD45 TUBE 9 I.36

  37. SSC SSC FLy CD64/CD24 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD24PE FSC-Height CD45Cy7 CD64FITC CD24PE CD24PE CD24PE CD64FITC CD64FITC CD64FITC I.37

  38. SSC SSC FLy CD64/CD10 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD10Cy5 FSC-Height CD45Cy7 CD64FITC CD10Cy5 CD10Cy5 CD10Cy5 CD64FITC CD64FITC CD64FITC I.38

  39. SSC SSC FLy CD24/CD10 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD10Cy5 FSC-Height CD45Cy7 CD24PE CD10Cy5 CD10Cy5 CD10Cy5 CD24PE CD24PE CD24PE I.39

  40. CD7/CD117/CD34/CD45 TUBE 10 I.40

  41. SSC SSC FLy CD7/CD117 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD117PE FSC-Height CD45Cy7 CD7FITC CD117PE CD117PE CD117PE CD7FITC CD7FITC CD7FITC I.41

  42. SSC SSC FLy CD7/CD34 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD34Cy5 FSC-Height CD45Cy7 CD7FITC CD34Cy5 CD34Cy5 CD34Cy5 CD7FITC CD7FITC CD7FITC I.42

  43. SSC SSC FLy CD117/CD34 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD34Cy5 FSC-Height CD45Cy7 CD117PE CD34Cy5 CD34Cy5 CD34Cy5 CD117PE CD117PE CD117PE I.43

  44. CD22/CD10/CD20/CD45 TUBE 11 I.44

  45. SSC SSC FLy CD22/CD10 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD10PE FSC-Height CD45PC7 CD22FITC CD10PE CD10PE CD10PE CD22FITC CD22FITC CD22FITC I.45

  46. SSC SSC FLy CD22/CD20 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD20PC5 FSC-Height CD45PC7 CD22FITC CD20PC5 CD20PC5 CD20PC5 CD22FITC CD22FITC CD22FITC I.46

  47. SSC SSC FLy CD10/CD20 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD20PC5 FSC-Height CD45PC7 CD10PE CD20PC5 CD20PC5 CD20PC5 CD10PE CD10PE CD10PE I.47

  48. CD4/CD2/CD56/CD45 TUBE 12 I.48

  49. SSC SSC FLy CD4/CD2 FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD2PE FSC-Height CD45APC CD4FITC CD2PE CD2PE CD2PE CD4FITC CD4FITC CD4FITC I.49

  50. SSC SSC FLy FSC FLx CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height CD56PC7 FSC-Height CD45APC CD4FITC CD56PC7 CD56PC7 CD56PC7 CD4FITC CD4FITC CD4FITC CD4/CD56 I.50

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