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Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA

CONFERENCE ON NANO-SAFETY 22.-24. April 2009, Ljubljana, Slovenia. Nanosized ZnO and nano TiO 2 : bioavailability and bioaccumulation. Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA E-mail: Damjana.Drobne@ bf. uni-lj.si.

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Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA

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  1. CONFERENCE ON NANO-SAFETY 22.-24. April 2009, Ljubljana, Slovenia Nanosized ZnO and nano TiO2: bioavailability and bioaccumulation Damjana DrobneUniversity of Ljubljana, Biotechnical Faculty Department of Biology, SLOVENIA E-mail:Damjana.Drobne@bf.uni-lj.si

  2. Research group for nanobiology and nanotoxicology Dept. Biology, Biotechnical faculty, Uni Ljubljana Damjana Drobne; Vladka Lešer; Janez Valant; ŽivaTkalec Pipan; Marjetka Kralj Kunčič; Sara Novak, student; Tea Romih; student ______________________________________________ National Institute of chemstry Anita Jemec ______________________________________________ Faculty for computer and information science, Uni Ljubljana Jernej Zupanc

  3. J1-9475 (C) Elaboration and evealution of a single species toxicity test for nanoparticles with a terrestrial isopod evaluation and Izdelava in vrednotenje testa strupenosti (basic research project), Slovenian Research Agency 2006-2009

  4. Aim of work …. to study interactions between nanoparticles and biological systes

  5. In vivo and in vitro biological system Lipid vesicles A terrestrial isopod, a model invertebrate organism

  6. Our past experiences ... studding toxicity of metal pollutants and pesticides present in the environment.

  7. Present challanges Interactions between nanoparticles and biological systems.

  8. + Photo: M. Bele, Institute of chemistry, Ljubljana .. .biological potential of nanosized metal oxides

  9. Chemicalsaffect target molecules or interfere with different intracellular compartments.The effect is propagated upwards to higher levels of biological complexity (cells, tissue, organs, organism) and it is manifested as toxic response.

  10. What about nanoparticles?

  11. 1) One hypothesis is that nanoparticles interact firstwith cell membrane! A consequesnce is….

  12. Photo: J. Valant … cells are affected, but nanoparticles were not detected inside the cells (nanoTiO2).

  13. 2) The other opened question is: are nanoparticle bioavailable; do they bioaccumulate? ? By L.Ziccardi, M. McArdle, Y. Lowney, J. Tsuji

  14. Do ZnO nanoparticles accumulate?

  15. Our work • model organism, terrestrial isopod P. scaber • food exposure to nanoparticles for 4 weeks • comparison among bioaccumulation of • nano ZnO, • macropowder ZnO • ZnCl2

  16. Background • terrestrial isopods accumulate high amounts of meals (Zn, Pb, Cu, Fe etc). in their tissue • a lot of knowledge already exist on the accumulation pattern and accumulation strategy of metals if offered as soluble salts

  17. Experimental set up Four weeks feeding on Nanoparticel, macropowsder or ZnCl dosed food + metal analyses in whole organism by AAS concentrations of Zn in the body

  18. Background knowledge: FIB/SEM,

  19. Photo: F.Tatti, FEI

  20. Scanning electron micrograph of macropowder of ZnO. Scanning electron micrograph of nanosized ZnO

  21. Results • no diffrences in amount of accumulated Zn among groups

  22. 5000 µg/g 2000 µg/g By Ž. Pipan Tkavec, D. Drobne, A.Jemec ,T.Romih, P.Zidar, M.Bele

  23. Discussion • we are not able to answere the question about nanoaprticle bioavailability or bioaccumauation pottential • the fact is that when nanosized or macrosized ZnO is ingetsed an equal prtion of Zn is accumulated as when ZnCl2 is inegested

  24. Present/Futuredirection of research • investigation of accumulation of other nanparticles with lower dissolution rates; for example Cu and Ag nanoparticles • Investigation of interaction between membranes and nanoparticles

  25. Photo by J.Zupanc Computer aided analysis of lipid vesicles shape transformations after incubation with nanoparticles.

  26. Publications: • DROBNE, Damjana, JEMEC, Anita, PIPAN, Živa. In vivo screening to determine hazards of nanoparticles: nanosized TiO2. Environ. pollut. ,2009, issue 4, vol. 157, str. 1157-1164 • JEMEC, Anita, DROBNE, Damjana, REMŠKAR, Maja, SEPČIĆ, Kristina, TIŠLER, Tatjana. Effects of ingested nano-sized titanium dioxide on terrestrial isopods Porcellio scaber. Environ. toxicol. chem., 2008, vol. 27, no. 9, str. 1904-1914, • JEMEC, Anita, DROBNE, Damjana, TIŠLER, Tatjana, SEPČIĆ, Kristina. Biochemical biomarkers in environmental studies-lessons learnt from enzymes catalase, glutathione S-transferase and cholinesterase in two crustacean species. Environ. sci. pollut. res. int., 2009, 11 str.,

  27. Thank you for your attention!

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