whole exome sequencing service

1. Whole Exome Sequencing Human genome comprises approximately 3 X 109 bases, and contains approximately 180,000 coding regions (exome), constituting about 1.7% of a human genome. It is estimated that 85% of the disease-causing mutations occur in the exome. For this reason, sequencing of the whole exome has the potential to uncover higher yield of relevant variants at a far lower cost than whole genome sequencing. Whole exome sequencing is thought to be an efficient and powerful way to identify the genetic variants that affect heritable phenotypes, including important disease-causing mutations and natural variations that can be used to improve crops and livestock. Whole Exome Sequencing utilizes exome capture technology to enrich exons , and then sequences these regions in a high-throughput manner. To be specific, DNA samples are first fragmented and biotinylated oligonucleotide probes (baits) are used to selectively hybridize to exome in the genome. Magnetic streptavidin beads are then used to bind to the biotinylated probes. The non- targeted portion of the genome is washed away, and the PCR is used to enrich the sample for DNA from the target region. Subsequently, the sample is sequenced by the Illumina HiSeq platform. This strategy can result in up to a 100-fold improvement in gene coverage for the human genome. The validated sequencing data are then used for variant analysis and clinical statements. Learn more about whole exome sequencing service at: https://www.cd-genomics.com/Whole-Exome-Sequencing.html