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Sensing DNA Damage Through ATRIP Recognition of RPA-ssDNA Complexes

Sensing DNA Damage Through ATRIP Recognition of RPA-ssDNA Complexes. Science 300 , 1542. Nucleic. Acids. Res. 34 , 4126-4137 (2006). ATR colocalizes with RPA to nuclear foci after DNA damage. 96%. 27%. The recruitment of ATR and ATRIP to the damage-induced

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Sensing DNA Damage Through ATRIP Recognition of RPA-ssDNA Complexes

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  1. Sensing DNA Damage ThroughATRIP Recognition of RPA-ssDNA Complexes Science300, 1542

  2. Nucleic. Acids. Res. 34, 4126-4137 (2006) ATR colocalizes with RPA to nuclear foci after DNA damage

  3. 96% 27% The recruitment of ATR and ATRIP to the damage-induced nuclear foci is dependent upon RPA.

  4. HU HeLa

  5. U2OS RPAregulates activation of ATR respond to not only replication blocks but also DNA damage.

  6. RPA confers upon ATRIP a higher affinity to ssDNA than to dsDNA or to the ends of dsDNA

  7. The binding of ATRIP to RPA-coated ssDNA is length-dependent with a threshold length between 50 and 75 not that renders ATRIP binding more efficient. EMBO J. 20, 612-618 (2001)

  8. The affinity of ATR for ssDNA is obscuredin the ATR-ATRIP complex The vast majority of ATR is present in complexes with ATRIP in human cells

  9. ATR-ATRIP RPA Rad17-Rfc-5 Recruitment of ATR-ATRIP to ssDNA can lead to its activation toward its substrates on DNA

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