Biotechnology and Genetic Engineering

1. Biotechnology and Genetic Engineering

2. _______________________________ • We can now grow new body parts and soon donating blood will be a thing of the past, but will we go too far? Photo of a mouse growing a "human ear"

3. Genetic Engineering • The human _____________________________________ of a cell. • ______________________________ - Genetically engineered DNA prepared by splicing genes from one species into the cells of a different species. Such DNA becomes part of the host's genetic makeup and is replicated.

4. genetically engineered tobacco plant with a __________________________

5. Recombinant DNA • The ________________________________ _______________________________ ex. Bacterial DNA + human gene for insulin production Tobacco Plant DNA + firefly DNA for bioluminescence plant

6. Forming Recombinant DNA Desired Gene DNA from target organism Recombinant DNA with gene of interest

7. Golden Rice • Golden rice is to develop rice varieties that _________________ (beta-carotene) as a means of alleviating vitamin A deficiencies in the diets of disadvantaged people in developing countries. (traditional rice varieties do not produce provitamin-A).

8. Growth hormone and therapy • ________________ controls several complex physiologic processes, including growth and metabolism. • Two baby mice - same age • Human Growth hormone gene inserted into the embryo of the mouse on the right causing rapid growth • The mouse on the left is a normal sized mouse

9. Insect Resistance • 'Bt' is an ______________________, marketed worldwide for control of many important _______________ - mainly caterpillars of the Lepidoptera (butterflies and moths), mosquito larvae, and black flies that vector river blindness in Africa. Results of insect infestation on Bt (right) and non-Bt (left) cotton balls.

10. Vaccines • Banana is currently being considered as an __________ ___________________________ • An edible vaccine doesn't need sterile syringes, costly refrigeration, or multiple injections • WHO finds more than 2 million children die worldwide each year from diarrhea caused by Escherichia coli and Vibrio cholara bacteria can be prevented easily with vaccines.

11. Land Mines • Neal Stewart at the University of North Carolina is developing ______________________ ______________________ • Plants could be ideal biosensors for land mines as seeds would be spread widely and evenly in a suspect field • The gene that can announce the presence of land mines is GFP

12. GFP and mice

13. Glo fish • Fluorescent zebra fish were specially bred to help ________________ _____________________. By adding a natural fluorescence gene to the fish, scientists are able to quickly and easily determine when our waterways are contaminated

14. Genetic Engineering • Genetic engineering techniques are used in a variety of industries, in agriculture, in basic research, and in medicine. This genetically engineered cow __________________ and can help to increase dairy production.

15. Genetic Engineering • There is great potential for the development of useful products through genetic engineering • EX., ________________________________________________ ____________________________________________________ Seedless watermelons are genetically engineered

16. Ethical & Moral Concerns • ___________is the production of ________________ cells or organisms.

18. Ethical & Moral Concerns • ___________ was the world's first cloned mammal. Two calves cloned from cells in cow's milk, Japan. Dolly the sheep 1996 - 2003

19. Gene Therapy • Gene therapy is a technique for ___________________________ _________________________________ for disease development. • Possible cures for: • diabetes • cardiovascular disease • cystic fibrosis • Alzheimer's • Parkinson’s • and many other diseases is possible.

20. Is it right to use cloning to create an entirely new human being? • Is it ethical to create an embryonic copy of John Doe to supply cells to keep John alive? • Does a multicellular ball of tissue -- an embryo -- have the same rights and status as a human being?