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Komponen Permukaan Sel

Biologi Sel - pertemuan IV. Komponen Permukaan Sel. Dr.DWI WINARNI, M.Si Dept. Biologi FSaintek Univ. Airlangga. Ligan terlarut ( soluble ligand ) Hormon , growth factor. MENENTUKAN BENTUK/JENIS. Non SELULAR (ECM). INTERAKSI. SEL HIDUP. Matriks ekstrasel ( fixed ligand ).

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Komponen Permukaan Sel

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  1. BiologiSel - pertemuan IV KomponenPermukaanSel Dr.DWI WINARNI, M.Si Dept. Biologi FSaintek Univ. Airlangga

  2. Liganterlarut (soluble ligand) Hormon, growth factor MENENTUKAN BENTUK/JENIS Non SELULAR (ECM) INTERAKSI SEL HIDUP Matriksekstrasel (fixed ligand) LINGKUNGAN SELULAR Komponenpermukaansel biosel_S1_bio

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  4. Komponenpermukaansel YANG BERPERAN DALAM INTERAKSI SEL DENGAN LINGKUNGANNYA 1. INTEGRINS 2.SELECTINS 3. IMMUNOGLOBULINS superfamily 4. CADHERINS biosel_S1_bio

  5. 1. INTEGRIN Merupakankelompok protein integral membran yang terdapatpadapermukaansel vertebrata • Tersusunatas 2 rantaipolipeptida, 1 rantaiadan 1 rantaib yang terangkaisecaranonkovalen • 16 jenisrantaia • 8 jenisrantaib 22 heterodimer biosel_S1_bio

  6. Rantaia meningkatkanspesifitaspengikatanligan biosel_S1_bio

  7. Integrin 1. ADESI SEL PADA SUBSTRAT/ SEL 2. TRANSMISI SINYAL DARI EKSTRASEL (outside-in signaling) biosel_S1_bio

  8. SEBAGIAN BESAR PROTEIN EKSTRASELULAR BERIKATAN DENGAN INTEGRIN PADA BAGIAN YANG MENGANDUNG URUTAN (SEKUENS) ASAM AMINO arginin-glisin-asamaspartat (nomenklaturbaru= RGD), denganligand -binding site tergantungpadakeberadaan ion Ca2+ atau Mg2+ biosel_S1_bio

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  10. BEBERAPA JENIS RESEPTOR INTEGRIN DAN JENIS LIGAN YANG DIKENAL MELALUI SEKUENS RGD biosel_S1_bio

  11. BEBERAPA JENIS RESEPTOR INTEGRIN DAN JENIS LIGAN YANG DIKENAL MELALUI SEKUENS nonRGD biosel_S1_bio

  12. biosel_S1_bio

  13. INTEGRIN MENGIKATKAN SEL PADA SUBSTRAT biosel_S1_bio

  14. FOCAL CONTACT / FOCAL ADHESIONS Seldalambiakansecaradiskritmengadakanperlekatanpadapermukaan. Kontakintegrin (a5b1) dengan protein disubstrat yang melapisipermukaan (mis: laminin, kolagen, fibronektin)  perubahankonformasiintegrinsitoplasmik perlekatanintegrindenganfilamenaktin  pengelompokan (clustering) integrindipermukaan biosel_S1_bio

  15. LM= laminin biosel_S1_bio

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  18. Focal contact merupakanstruktur yang dinamik, yang setelahterbentuk, secaracepatakan “terurai” jikaseldalambiakanterstimulasiuntuk mitosis atauperubahanorganisasisitoskeleton yang lain biosel_S1_bio

  19. HEMIDESMOSOM biosel_S1_bio

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  21. Seldapatmengekspresikanberbagaijenisintegrinpadapermukaannya Seldapatmengikatberbagaijeniskomponenmatriksekstraselular biosel_S1_bio

  22. Sel basal epidermis merupakansatu-satunyajenisseldalamlapisanepitel epidermis yang mempunyaikemampuanmembelah, jikasintesisintegrintidakberhentisaatselmeninggalkan basal selakanterusmembelah kondisimirip psoriasis (epidermis menebaldanmengalamiperadangan) Sel-sel basal mengekspresikanintegrina2b1, a3b1dana5b1 yang berperandalamikatandesmosomdengankomponenmembranabasalis

  23. Bullouspemphigoid disebabkanolehterbentuknyaautoantiboditerhadapstrukturhemidesmosom selepitelbagian basal tidakdapatmelekatpadamembranabasalisdanjaringanikatdibawahnya  terisicairantubuh bula, terutamanampakpadakulit Epidermolysisbulosa disebabkanolehfaktorgenetis (perubahanpada protein penyusunhemodesmosomtermasuk subunit a6ataub4 integrinataulaminin  bula (termasukpadadindingsaluran gastrointestinal danurin) biosel_S1_bio

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  31. 2. SELEKTIN (selectinS) Merupakankelompokglikoprotein integral membran yang dapatmengenalidanmengikatgugusgulatertentu yang terdapatpadapermukaansel lain Namaselectinberasaldarilectinyaitusenyawa yang dapatmengikatgugusgulatertentusecaraspesifik pengikatanselektinpadaligannyamemerlukan ion kalsium biosel_S1_bio

  32. E-SELECTINS CD62e SEL-SEL ENDOTEL PLATELETS ENDOTEL P-SELECTINS CD62p SELECTINS SEMUA JENIS LEKOSIT L-SELECTINS CD62l biosel_S1_bio

  33. Lymphocytes homing biosel_S1_bio

  34. T cell–endothelial-cell interactions as studied in flow chamber systems in vitro. P-selectin-dependent interactions require functional P-selectin glycoprotein ligand 1 (PSGL1); E-selectin interactions require poorly defined E-selectinligand(s) on activated T cells. L-selectin on T cells interacts with peripheral node addressin (PNAD). At sites of inflammation, endothelial cells express P- and E-selectin and during chronic inflammation PNAD is also expressed by endothelial cells biosel_S1_bio

  35. At sites of inflammation, neutrophils and monocytes, or neutrophil- or monocyte-derived microparticles, interact with the inflamed endothelium and present functional PSGL1 to T cells. This PSGL1 can interact with L-selectin on naive or central memory T cells. Activated platelets and platelet-derived microparticles are also known to interact with the vascular endothelium and can present P-selectin to T cells. Note that for simplicity, not all domains of the selectins are depicted, although they are represented according to their relative sizes. biosel_S1_bio

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  38. 3. Immunoglobulin superfamily biosel_S1_bio

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  41. A schematic of the structure of the T-cell receptor (TCR)

  42. Antigen presentation stimulates T cells to become either "cytotoxic" CD8+ cells or "helper" CD4+ cells

  43. The low-affinity IgE receptor, Fc RII (CD23), is expressed by a wide variety of immune cell types, including B cells and dendritic cells. IgE that is bound to Fc RI or Fc RII can facilitate allergen uptake by antigen-presenting cells (APCs) and augment secondary immune responses. b | Most IgE is bound by its high-affinity receptor, Fc RI, expressed by mast cells and basophils. Crosslinking of IgE bound to Fc RI on tissue mast cells by specific antigen results in the local release of inflammatory mediators (for example, histamine and leukotrienes), enzymes and cytokines that mediate the clinical manifestations of atopy. biosel_S1_bio

  44. Neural cell adhesion molecule (NCAM) and L1 have important roles in cell–cell interactions through homophilic (NCAM–NCAM or L1–L1) and heterophilic (NCAM–L1) binding. They affect neurocircuitry (panel b), and, by interacting with cytoskeletal components, they can activate specific intracellular signalling pathways. These molecules participate in neurite extension and guidance, cell differentiation and survival, and synaptogenesis. In addition to their pivotal roles in neural development and regeneration, they have been strongly implicated in synaptic plasticity and memory formation biosel_S1_bio

  45. 4. CADHERINS • DITEMUKAN SECARA LUAS DI PERMUKAAN SEL • UMUMNYA DIMER • BAGIAN EKSTRASEL MERUPAKAN STRUKTUR TANDEM BERULANG 5 • BAGIAN INTRASEL BERIKATAN DENGAN PROTEIN SITOPLASMIK CATENIN CATENIN BERIKATAN DENGAN AKTIN SITOSKELETON • Jenis: • 1. yang berinteraksidengansitoskeleton • (Cadherin N, P, R, B, dan E) • 2. berasosiasidengandesmosom (desmoglein, desmocolin) biosel_S1_bio

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  48. Peranchaderindalammorfogenesis The epithelial–mesenchymal transition (EMT) and its reverse — the mesenchymal–epithelial transition. E-cadherin (epithelial cadherin) mediates the adhesion of cells, the transition of cells from organization in a loose mesenchymal network — in which they lack polarity and have migratory and invasive potential — to their tight apposition in a polarized epithelial barrier, and the formation of tight junctional complexes. Loss of E-cadherin expression is associated with the EMT and its loss or dysregulation plays a part in tumour cell invasion and metastasis. The yellow cells express E-cadherin, in contrast to the blue cells that do not biosel_S1_bio

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