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Antimicrobial Properties of Garfish Blood

Antimicrobial Properties of Garfish Blood. Rachel E. Laird Nicholls State University. Antimicrobial Studies Involving Fish Blood Rainbow trout ( Oncorhynchus mykiss ) model.

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Antimicrobial Properties of Garfish Blood

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  1. Antimicrobial Properties of Garfish Blood Rachel E. Laird Nicholls State University

  2. Antimicrobial Studies Involving Fish Blood Rainbow trout (Oncorhynchus mykiss) model. Hollebecq et al;1995; - complement blood plays a significant role in defending against infection, the bactericidal activity of the trout blood is more accurate in defending against furunculosis. Bandin et al.; 1995; - opsonized bacteria increased their survival inside phagocytes Nikoskelainen et al.;2002; - rainbow trout complement activity increased from 10oC to 35oC

  3. Why Gar Blood? • There have been no studies to date on the effects of gar blood on bacterial activity. • Help gain a better understanding of garfish biology. • Establish a foundation to further explore blood components of an ancient fish species. • Isolating and characterizing the molecules would help aid in a better understanding of host-pathogen interactions and pathways.

  4. Background Fish immune responses against bacteria involve both cellular and non-cellular components Complement and small antimicrobial peptides are thought to be important innate immune responses Activity of blood is dependent upon such factors as temperature. Bayou Lafourche has 106 CFU/ml Aerobic bacterial load (Nathaniel, R. unpublished)

  5. Complement –series of enzymatic events resulting in formation of a ‘membrane attack complex, ‘death by a hole puncher’ Antimicrobial Peptides – cationic peptides, 5-60 aa, disrupt membrane integrity.

  6. Objective: To determine the antimicrobial properties in Garfish Blood. Hypotheses: • Gar blood will have a bactericidal effect. • Bactericidal effect will follow a dose dependent and time dependent mechanism.

  7. Audubon Bridge Water Collection Point

  8. Satellite Image of Mississippi Drainage Basin New Orleans Thibodaux Houma

  9. Methods Bayou Lafourche Water Control = Medium + Bayou water Serial dilutions Test = Medium + Bayou water + Blood Blood Sterility Control = Medium + Blood All results incubated at 37°C for 18 hrs, OD600 readings taken

  10. Effects of Gar Blood on Bacterial Growth Bacterial Number CFU/ml Bacterial Number CFU/ml

  11. Mean Effects of Gar Blood on Bacterial Growth * Bacterial Number (CFU/ml) * N=4; *P<0.001

  12. Effects of Gar Blood on Bacterial Growth over 24 Hours Bacterial Number CFU/ml N=6

  13. Effect of Heat Treated Gar Blood on Bacterial Growth Bacterial Number CFU/ml

  14. Mean Effects of Heat Treated Gar Serum on Bacterial Growth Bacterial Number CFU/ml N=3

  15. Summary Gar blood (@10%) showed antimicrobial activity against up to ~1,000 bacteria/ml We did not yet find antimicrobial activity to follow a time dependent mechanism. We have not yet been able to show antimicrobial activity to be heat labile.

  16. Acknowledgements Dr. Quenton Fontenot Dr. Allyse Ferrara Mr. Rickey Verrett (Gar Fisherman) Ms. Olivia Smith Dr. Rajkumar Nathaniel Funded by: LA Board of Regents

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