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Triage of Women with ASCUS and ASC-H Cytology by PAX1 Methylation

Triage of Women with ASCUS and ASC-H Cytology by PAX1 Methylation. XIV-029. 1 . 三軍總醫院病理部暨國防醫學院病理研究所 2 . 三軍總醫院婦產部 柯鋒翌 ＊ 1 、 賴鴻政 ＃ 2 、 趙載光 ＃ 1. Background. Results. PAX1 methylation test. Follow -up Histology. Previous Cytology. N=56.

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Triage of Women with ASCUS and ASC-H Cytology by PAX1 Methylation

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  1. Triage of Women with ASCUS and ASC-H Cytology by PAX1 Methylation XIV-029 1.三軍總醫院病理部暨國防醫學院病理研究所 2.三軍總醫院婦產部 柯鋒翌＊1、賴鴻政＃2、趙載光＃1 Background Results PAX1 methylation test Follow -up Histology Previous Cytology N=56 The identification of a small percentage of carcinoma in situ (CIS) among patients with minor cytological abnormalities (atypical squamous cells of undetermined significance [ASCUS] or atypical squamous cells cannot exclude HSIL [ASC-H] group) is a major problem in cytology-based cervical cancer screening. Here we investigated the efficacy of PAX1 methylation as a biomarker to identify samples of patients with CIS among those with an ASCUS or ASC-H result in Papanicolaou cytology. Follow-up diagnosis TestResult A B (+)=7 (-)=49 (+)=1 Reactive Change (-)=49 ASCUS ASCUS (+)=6 Carcinoma In situ C D (-)=0 Sensitivity =100% Specificity = 98% E F Materials and Methods Conclusion Consecutive liquid-based cytology specimens of 40 ASCUS and 10 ASC-H results were selected from gynecologists who subsequent follow-up with biopsy or repeat smear 6 months later, independent of the PAX1 result. Genomic DNA was extracted from liquid-based smears and then treat with bisulfite to converse non-methylation cytosine. After that we use real-time polymerase chain reaction (PCR) to detect PAX1 and COL2A1. To measure of gene methylation we calculated the “percentage of methylated reference” (PMR) for each real-time PCR result using Ct values for the PAX1 gene and a control COL2A1 gene. CIS These data suggested that the use of PAX1 methylation state as a biomarker in cervical cytology to triage ASCUS and ASC-H cases allows identification of CIS with good sensitivity and specificity. Fig.1 (A)cytology diagnosis with ASCUS. (B)Normal cervical epithelial (C) cytology diagnosis with ASC-H. (D)Squamous metaplasia. (E)Carcinoma in situ . (F) Carcinoma in situ. References ASC-H ASCUS • Gynecologic Oncology 2003, 91:201-208. • 2.Cancer Cytopathol 2004; 102:100-108. • 3.Cancer Cytopathol 2007;111:58–66. • 4.Int. J. Cancer: 2008;123, 161–167 . CIS Reactive change CIS Reactive change 5 1 10 Case number 40

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