1 / 14

The Identification and Characterization of the Cardioactive Principals in Hawthorn Extract

The Identification and Characterization of the Cardioactive Principals in Hawthorn Extract . by Niel Henriksen Mentor: Dr. Phil Proteau College of Pharmacy. Hawthorn and Heart Failure.

lindley
Download Presentation

The Identification and Characterization of the Cardioactive Principals in Hawthorn Extract

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. The Identification and Characterization of the Cardioactive Principals in Hawthorn Extract by Niel Henriksen Mentor: Dr. Phil Proteau College of Pharmacy

  2. Hawthorn and Heart Failure • Congestive heart failure (CHF) results from coronary artery disease, a previous heart attack, high blood pressure, heart defects, and infections in the heart tissue. • Nearly 5 million people in the United States are diagnosed with CHF. • The symptoms of CHF include shortness of breath, fatigue, swelling and kidney dysfunction.

  3. Hawthorn Extract Benefits • Clinical trials by the NYHA demonstrated: (+) exercise tolerance (-) dyspnea (shortness of breath) (-) ankle swelling (-) blood pressure • Studies on cardiac tissue show: (+) inotropic effects (+) cardiac flux (+) vasorelaxation (+) anti-arrhythmic potential

  4. Hawthorn Extract Unknowns • Active compound(s) • Flavonoids and Procyanidins? • Mechanism of action • Proper dosing • Duration of therapy • Most effective extract source (berries, leaves, or flowers)

  5. Project Goals • Develop cell based bioassays for evaluation of the direct cardiac effects of hawthorn extract • Using bioassay-guided fractionation, identify the components in hawthorn extract with cardiac activity

  6. Bioassay Tests • Rate of Contraction • Amplitude of Contraction

  7. Isolation Techniques • Solvent Extraction • Thin-Layer Chromatography • Large Scale Chromatography • High Performance Liquid Chromatography

  8. Identification Techniques • Mass Spectrometry • Nuclear Magnetic Resonance (NMR) Spectroscopy

  9. Future Goals • Complete bioassay-guided fractionation • Determine the most effective extract source • Determine the mechanism through with hawthorn extract functions • Correlate the plasma levels of cardioactive component with the physiologic effects on animal models

  10. Acknowledgements • Dr. Phil Proteau • Shannon Long • Dr. Theresa Filtz • Dr. Kevin Ahern • Howard Hughes Medical Institute

More Related